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背景高原低氧条件下生物体内反应性氧族产生增加,烟酰胺腺嘌呤二核苷磷酸(NADPH)氧化酶作为反应性氧族产生的主要酶,在高原高血压的发生发展中具有主导作用。目的探讨不同海拔低氧对高血压患者血清NADPH氧化酶、钙/钙调蛋白激酶Ⅱ(CaMKⅡ)水平的影响。方法入选汉族原发性高血压(EH)患者175例,其中A组:居住于海拔2260～2295m患者130例,包括高血压1级(EH1)40例,高血压2级(EH2)46例,高血压3级(EH3)44例;B组:居住于海拔>3000m高血压3级(EH3)患者45例。选取同期居住在海拔2260～2295m的健康体检人群43例(对照组)。使用酶联免疫吸附(ELISA)法测定静脉血清NADPH氧化酶、CaMKⅡ浓度。结果海拔2260～2295m随着血压级别的增加,NADPH氧化酶水平逐渐增高,且高于对照组[EH1(331.5±83.9)、EH2(361.4±139.6)、EH3(521.9±171.4)比对照组(256.2±154.8)ng/L,均P<0.05],EH3较EH1、EH2增高(P<0.05);海拔>3000mEH3NADPH氧化酶水平较海拔2260～2295mEH3增高[(795.6±411.7)比(521.9±171.4)ng/L,P<0.05]。海拔2260～2295m:CaMKⅡ浓度EH3高于对照组、EH1、EH2[(18.2±8.1)ng/L比(5.2±3.0)、(6.3±2.2)、(6.6±3.1)ng/L,均P<0.05];海拔>3000mEH3CaMKⅡ浓度较海拔2260～2295mEH3略高,但差异无统计学意义[(22.6±8.3)比(18.2±8.1)ng/L,P>0.05]。结论高原地区高血压患者NADPH氧化酶水平高于正常人,随血压分级的增高而增高;高海拔高血压3级患者NADPH氧化酶水平高于较高海拔同级患者。
In the background of plateau hypoxia, reactive oxygen species in organisms increased, nicotinamide adenine dinucleoside phosphate (NADPH) oxidase as reactive oxygen species in the production of the main enzyme in the development of high altitude hypertension plays a leading role. Objective To investigate the effects of different altitude hypoxia on serum levels of NADPH oxidase and calcium / calmodulin Ⅱ (CaMKⅡ) in patients with essential hypertension. Methods One hundred and seventy-five patients with essential hypertension (EH) were enrolled in this study. Group A: 130 patients (range, 2260 to 2295m), including 40 with grade 1 hypertension (EH1), 46 with grade 2 hypertension (EH2) Forty-four patients with grade 3 hypertension (EH3) and group B (45 patients) with hypertension at grade 3 hypertension (EH3). Select the same period living in the elevation of 2260 ~ 2295m health examination population 43 cases (control group). Serum NADPH oxidase and CaMKII concentrations were measured by enzyme-linked immunosorbent assay (ELISA). Results Elevation of 2260 ~ 2295m increased with the increase of blood pressure level, the level of NADPH oxidase was higher than that of control group [EH1 (331.5 ± 83.9), EH2 (361.4 ± 139.6), EH3 (521.9 ± 171.4) ± 154.8 ng / L, all P <0.05]. EH3 levels were significantly higher than those of EH1 and EH2 (P <0.05). Elevation of 3000mEH3NADPH oxidase was higher than that at altitude of 2260-2295 mEH3 [(795.6 ± 411.7) vs 521.9 ± 171.4 /L,P<0.05]. Elevation of 2260 ~ 2295m: the concentration of CaMKⅡelement EH3 was higher than that of the control group, EH1 and EH2 were (5.2 ± 3.0), (6.3 ± 2.2) and (6.6 ± 3.1) ng / 0.05]. Elevation> 3000mEH3CaMKⅡ was slightly higher than that of 2260 ~ 2295mEH3, but the difference was not statistically significant (22.6 ± 8.3 vs 18.2 ± 8.1 ng / L, P> 0.05). Conclusion The level of NADPH oxidase in hypertensive patients in the plateau is higher than that in normal subjects and increases with the increase of blood pressure. The level of NADPH oxidase in patients with high grade hypertension is higher than that in patients with higher altitude.