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目的:建立不同产地古丽娜的HPLC指纹图谱,考察不同产地古丽娜药材的质量。方法:采用Venusil XBP C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈(A)-0.4%磷酸溶液(B),梯度洗脱,流速为1.0 mL/min;柱温:25℃;检测波长:280 nm;进样量10μL。结果:确定由12个共有峰构成古丽娜药材的指纹特征,精密度和重复性中共有峰相对保留时间和相对峰面积RSD均小于3.0%,12批样品与生成的对照指纹图谱的相似度均大于0.85。对12批药材进行聚类分析,分成3类,聚类分析显示产地和采集时间与药材的质量相关。结论:该方法精密度高、重现性好,可为古丽娜药材的质量评价提供参考,也为古丽娜药材进一步开发利用奠定基础。
OBJECTIVE: To establish the HPLC fingerprint of Gulina in different habitats and investigate the quality of gulina in different habitats. Methods: Venusil XBP C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.4% phosphoric acid solution (B) with a gradient elution at a flow rate of 1.0 mL / ; Detection wavelength: 280 nm; injection volume 10μL. Results: The fingerprints of Gulina medicinal materials, which consisted of 12 common peaks, were identified. The RSDs of relative peak area and relative peak area in precision and reproducibility were all less than 3.0%. The similarity of 12 batches of samples to the control fingerprinted All greater than 0.85. Cluster analysis of 12 batches of herbs was divided into three categories, cluster analysis showed that the origin and collection time and the quality of herbs related. Conclusion: The method has high precision and good reproducibility, which can provide reference for the quality evaluation of Gulina medicinal materials and lay a foundation for the further development and utilization of Gulina medicinal materials.