论文部分内容阅读
目的观察重组改构肿瘤坏死因子(TNF-α)在体外和顺铂、吉西他滨联合对人肺腺癌H1299细胞系杀伤作用。方法MTT法检测不同浓度TNF-α、顺铂、吉西他滨单药对H1299细胞的抑制率,一定浓度的TNF-α与不同浓度顺铂或吉西他滨联合时对H1299细胞的抑制率,有或无TNF-α作用下H1299细胞的平板克隆形成率和细胞生长曲线,流式细胞仪检测各组细胞凋亡率。结果在不同浓度TNF-α作用下,H1299细胞抑制率均为负值,与药物浓度无明显相关性;TNF-α对H1299细胞的细胞生长曲线和克隆形成率均无明显影响;TNF-α与顺铂联合时在顺铂浓度为10μg/m l时H1299细胞抑制率,明显高于单纯顺铂的作用(P<0.05);TNF-α与吉西他滨联合时在吉西他滨浓度为20、40μg/m l时对H1299细胞抑制率明显高于单纯吉西他滨的作用(P<0.05)。流式细胞分析各组细胞凋亡率与MTT结果一致。结论在体外重组改构肿瘤坏死因子与顺铂或吉西他滨联合应用明显增强后者对H1299细胞的杀伤作用,具有化疗增敏的作用。
Objective To observe the cytotoxicity of recombinant TNF-α combined with cisplatin and gemcitabine on human lung adenocarcinoma H1299 cell line in vitro. Methods MTT assay was used to detect the inhibitory rates of H1299 cells treated with different concentration of TNF-α, cisplatin and gemcitabine. The inhibitory rates of certain concentration of TNF-α combined with cisplatin or gemcitabine on H1299 cells with or without TNF- The formation rate and growth curve of H1299 cells were analyzed by flow cytometry. The apoptosis rate of H1299 cells was detected by flow cytometry. Results Under the action of different concentration of TNF-α, the inhibitory rate of H1299 cells was negative and had no significant correlation with the drug concentration. TNF-α had no significant effect on the cell growth curve and clonogenic rate of H1299 cells. TNF- Cisplatin combined with cisplatin at a concentration of 10μg / ml H1299 cell inhibitory rate was significantly higher than the effect of cisplatin alone (P <0.05); TNF-α and gemcitabine combination of gemcitabine concentration of 20,40μg / ml when The inhibitory rate of H1299 cells was significantly higher than that of gemcitabine alone (P <0.05). Flow cytometry analysis of apoptotic cells in each group and MTT results. Conclusion The combination of recombinant TNF-α and cisplatin or gemcitabine in vitro significantly enhances the killing effect of the latter on H1299 cells and has the effect of chemosensitization.