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采用溶剂法、硅胶柱色谱、ODS柱色谱和HPLC等手段对五指那藤Stauntonia obovatifoliola subsp.intermedia茎中的化学成分进行分离纯化,并通过NMR,MS等谱学方法进行结构鉴定。从五指那藤茎的60%乙醇提取物中的乙酸乙酯、正丁醇萃取部位中分离得到19个化合物,分别为羽扇豆醇(1)、白桦酮酸(2)、白桦脂酸(3)、3-表-白桦脂酸(4)、木通种酸(5)、24-O-乙酰木通种酸(6)、3-O-α-L-阿拉伯糖基-30-去甲常春藤皂苷元-28-O-α-L-鼠李糖基(1→4)-β-D-葡萄糖基(1→6)-O-β-D-葡萄糖基酯(7)、Stauntoside A(8)、刺楸皂苷A(9)、刺楸皂苷J(10)、Kizuta saponin K10(11)、3-O-α-L-鼠李糖基(1→2)-O-α-L-阿拉伯糖基常春藤皂苷元-28-β-D-木糖基(1→6)-O-β-D-葡萄糖基酯(12)、刺楸皂苷B(13)、3-O-α-L-鼠李糖基(1→2)-O-α-L-阿拉伯糖基-常春藤皂苷元-28-O-β-D-葡萄糖基(1→6)-O-β-D-葡萄糖基酯(14)、sieboldianoside A(15)、septemoside A(16)、刺楸皂苷K(17)、刺楸皂苷Ⅰ(18)、3-O-α-L-阿拉伯糖基(1→2)-O-β-D-葡萄糖醛酸基-常春藤皂苷元(19)。其中4,6,10,12,14,16~19为首次从该属植物中分得,且6为新的天然产物。
The chemical constituents of Stauntonia obovatifoliola subsp.intermedia stem were isolated and purified by solvent method, silica gel column chromatography, ODS column chromatography and HPLC. The structures were identified by NMR, MS and other spectral methods. Nineteen compounds were isolated from the ethyl acetate and n-butanol extraction fractions from the 60% ethanol extracts of the five-finger cane, including lupeol (1), birchkinolic acid (2), and betulinic acid (3). ), 3-tablet - betulinic acid (4), carnitine acid (5), 24-O-acetylacetonate (6), 3-O-α-L-arabinosyl-30- Heptosapogenin-28-O-α-L-rhamnosyl (1→4)-β-D-glucose (1→6)-O-β-D-glucosyl ester (7), Stauntoside A (8) Robinia pseudoacacia saponin A (9), Robinia pseudoacacia J (10), Kizuta saponin K10 (11), 3-O-α-L-rhamnosyl (1→2)-O-α-L - arabinosylhydantoin-28-β-D-xylosyl (1→6)-O-β-D-glucosyl ester (12), Robinia saponin B (13), 3-O-α -L-rhamnosyl (1→2)-O-α-L-arabinosyl-helioside-28-O-β-D-glucose (1→6)-O-β-D- Glucose ester (14), sieboldianoside A (15), septemoside A (16), Robinia saponin K (17), Robinia saponin I (18), 3-O-α-L-arabinosyl (1→2) )-O-β-D-glucuronosyl-hemodiagenin (19). Among them, 4,6,10,12,14,16~19 were isolated from this genus for the first time, and 6 is a new natural product.