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本文报道一种研究人体直肠中药物转运机制的灌流系统,用这种系统可对直肠壁的特定区域进行单次通过(以恒速灌流,药物浓度恒定)和重复循环灌流(以一定量灌流液重复循环,药物浓度逐渐降低)。该装置是由顶端为漏斗形的外管和顶端为一带孔小球的内管所构成,两管可以根据需要调节,使球和漏斗之间的距离为1～12cm。在漏斗和球上紧扎一透析袋(直径为2.9cm)。灌流液置37℃水浴中预热,用循环泵从外管输入透析袋中,再从内管的孔中排出(见图)。在单次通过实验中,灌流液通过溢流器和带有阀的刻度管
This article reports a perfusion system for studying the drug transport mechanism in the rectum of the human body. With this system, a single passage of a specific region of the rectal wall can be carried out (constant flow rate, constant drug concentration) and repeated perfusion Repeat the cycle, drug concentration gradually decreased). The device is composed of an outer tube with a funnel shape at the top and an inner tube with a small ball at the top. The two tubes can be adjusted according to needs so that the distance between the ball and the funnel is 1~12 cm. A dialysis bag (2.9 cm diameter) was tightened over the funnel and the ball. The perfusate was pre-warmed to 37 ° C in a water bath and circulated from the outer tube into the dialysis bag and expelled from the inner tube (see figure). In a single pass assay, the perfusate passes through an overflow and a graduated tube with a valve