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氯化烃类可损害细胞的线粒体。作者研究了急性二氯乙烷对肝脏线粒体氧化磷酸化的影响,以阐明毒物对肝细胞的作用机理。实验用向日葵油配成20%的二氯乙烷溶液,按0.7毫升/公斤剂量给体重200～250克雄性大鼠灌胃,对照组只给予同量的向日葵油。经过1、3或6天把动物处死,快速取出肝脏,称重,然后放入盛有7倍体积的0.25 M冷蔗糖水和pH 7.4的0.001 M 溶液的玻璃匀浆器中
Chlorinated hydrocarbons can damage the mitochondria of cells. The authors studied the effects of acute dichloroethane on oxidative phosphorylation of the liver mitochondria to elucidate the mechanism of toxic effects on hepatocytes. Experiment with sunflower oil dubbed 20% dichloroethane solution, according to 0.7 ml / kg dose to weight 200 ~ 250 g male rats gavage, the control group was given only the same amount of sunflower oil. The animals were sacrificed on days 1, 3, or 6, the livers removed rapidly, weighed and placed in a glass homogenizer containing 7 volumes of 0.25 M cold sucrose water and a 0.001 M solution of pH 7.4