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目的 通过对脐血和外周血免疫功能的比较 ,探讨了脐血移植中移植物抗宿主病(GVHD)低发生率的分子机理。方法 经增殖试验、NK杀伤活性试验及免疫荧光双标记方法研究了 4种血清对T细胞免疫功能的影响。结果 与成人血清相比较 ,脐血血清能降低NK细胞杀伤肿瘤靶细胞的活性 ;同时脐血血清和成人血清都能增加丝裂原特异性T淋巴细胞增殖 ,成人血清在增殖反应中可达到峰值 ,而脐血血清没有达到峰值效应 ,甚至在 0 1的稀释度时仍没有下降 ;成人血清能增加PHA刺激外周血单个核细胞 (PBMC)中的T淋巴细胞HLA DR表达 ,而脐血血清却没有这种效应。结论 血清中所分泌的某些可溶性因子可能在T细胞活化中起重要作用 ,与GVHD发生机理相关联
Objective To investigate the molecular mechanism of the low incidence of graft-versus-host disease (GVHD) in cord blood transplantation by comparing the immune function between cord blood and peripheral blood. Methods The effects of four kinds of serum on immune function of T cells were studied by proliferation assay, NK cytotoxicity assay and double immunofluorescence staining. Results Compared with adult serum, cord blood serum could reduce the activity of NK cells killing tumor target cells. Both cord blood serum and adult serum could increase the proliferation of mitogen-specific T lymphocytes, and the serum levels of adult serum peaked in proliferative response , While umbilical serum did not reach its peak value and did not decrease even at the dilution of 0 1. Adult serum increased HLA DR expression of T lymphocytes in peripheral blood mononuclear cells (PBMCs) stimulated by PHA, while serum from cord blood No such effect. Conclusion Some of the soluble factors secreted in serum may play an important role in the activation of T cells and are associated with the pathogenesis of GVHD