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以大黄素和大黄酚作为复方中成药有效成分的模型物，优化了用鲁米诺-H2O2－Cr3+作化学发光淬灭系统，测定大黄素和大黄酚的实验条件，以化学发光淬灭值作为大黄素和大黄酸的量度。测定的精密度和灵敏度都较高。含大黄复方中成药的提取液经TLC分离，分出大黄素和大黄酚用本法测定，结果与分光光度法一致。
Using emodin and chrysophanol as the active ingredients of the compound Chinese patent medicine, the chemiluminescence quenching system using luminol-H2O2-Cr3+ was optimized, and the experimental conditions for the determination of emodin and chrysophanol were optimized. The chemiluminescence quenching value was taken as Measurement of emodin and rhein. The precision and sensitivity of the assay are high. The extract containing rhubarb compound proprietary Chinese medicine was separated by TLC and the emodin and chrysophanol were separated and measured by this method. The results were consistent with the spectrophotometric method.