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目的 探讨养正散结汤对人胃癌癌前病变（PLGC）细胞增殖、凋亡及抑癌性miRNA表达的影响。方法 采用N-甲基-N′-硝基-N-亚硝基胍(MNNG)转化的人胃黏膜上皮细胞GES-1（MC细胞）作为PLGC细胞模型，以10%浓度的养正散结汤含药血清处理MC细胞，采用cck-8法检测细胞增殖，Annexin V-FITC/PI双染法流式细胞术检测细胞凋亡，实时定量PCR检测let-7a和miR-7的表达。结果 养正散结汤组48h、72h OD值均低于阴性对照组（P＜0.01），细胞凋亡率高于阴性对照组（P＜0.01）；MC细胞let-7a、miR-7的表达均低于GES-1细胞（P＜0.05）；经养正散结汤大鼠含药血清干预后，let-7a、miR-7的表达显著上调（P＜0.01）。结论 养正散结汤可能通过上调抑癌性miRNA let-7a、miR-7的表达而抑制MC细胞增殖、诱导其凋亡。
Objective To investigate the effects of Yangzheng Sanjie Decoction on proliferation, apoptosis and tumor suppressor miRNA expression in human gastric precancerous lesions (PLGC). Methods Human gastric mucosal epithelial cells GES-1 (MCs) transformed with N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) were used as a model of PLGC cells. The drug-containing serum was used to treat MC cells. The cell proliferation was detected by cck-8 assay. Apoptosis was detected by flow cytometry with Annexin V-FITC / PI double staining. The expression of let-7a and miR-7 were detected by real-time quantitative PCR. Results The OD values of Yangzheng Sanjie Decoction group at 48h and 72h were lower than that of negative control group (P <0.01), and the apoptosis rate of Yangzheng Sanjie Decoction group was higher than that of negative control group (P <0.01). The let-7a and miR- (P <0.05). The expression of let-7a and miR-7 was significantly up-regulated (P <0.01) after the intervention of Yangzheng Sanjie Decoction. Conclusion Yangzheng Sanjie decoction may inhibit the proliferation and induce the apoptosis of MC cells by up-regulating the expression of miRNA let-7a and miR-7.