论文部分内容阅读
断乳小鼠在饲料相同的基础上，两组动物分别喂以普通水和１％牛磺酸，于３６天（幼龄期）和１１０天（中龄期）时断头处死，测定其肝、脑匀浆的ＳＯＤ活性，ＭＤＡ及脂褐质含量，结果表明：补充牛磺酸的小鼠肝脑匀浆ＳＯＤ在幼龄期即显著高于对照组（Ｐ＜０．０５），至中龄期更为显著（Ｐ＜０．０１）。脑匀浆中ＭＤＡ及脂褐质含量在幼龄期时无差异，至中龄时，实验组含量显著低于对照组（Ｐ＜０．０１）。肝匀浆中脂褐质含量在不同鼠龄时与脑匀浆相同，但ＭＤＡ则不同鼠龄时无变化。本实验又以１１０天、１５０天、１８０天鼠龄之脾细胞进行细胞悬液培养后，测其ＤＮＡ经紫外线照射后的修复能力，结果证明，ＤＮＡ损伤修复能力随年龄增长而降低，但牛磺酸组在各年龄段的修复能力均显著高于对照组（Ｐ＜０．０１），说明牛磺酸在体内具有明显的抗氧化和延缓衰老的功能，并可能作用于ＤＮＡ分子水平。
On the basis of the same feed, the two groups of animals were fed normal water and 1% taurine respectively on weanling mice and decapitated at 36 days (young age) and 110 days (middle age), and their liver , SOD activity, MDA content and lipofuscin content in brain homogenate. The results showed that SOD in liver-brain homogenate supplemented with taurine was significantly higher in young mice than in control group (P <0.05) The age was more significant (P <0.01). There was no difference in MDA and lipofuscin content in brain homogenate at early age. At middle age, the contents of MDA and lipofuscin in experimental group were significantly lower than those in control group (P <0.01). Lipofuscin content in liver homogenates was the same as that in brain homogenates at different mouse ages, but MDA did not change at different mouse ages. In this experiment, spleen cells of 110 days, 150 days and 180 days of age were cultured in cell suspension to measure their DNA repair ability after UV irradiation. The results showed that DNA damage repair ability decreased with age, The repair ability of sulfonic acid group in all age groups was significantly higher than that of the control group (P <0.01), indicating that taurine has obvious antioxidant and anti-aging functions in vivo and may act on DNA molecular level.