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用遗传手段获得同质异核,异质同核的水稻(Oryzo satiue L.),提取大亚基(LS)相同、而小亚基(SS)不同和 LS 不同,但 SS 相同的核酮糖-1,5-二磷酸羧化酶/加氧酶(RubisCO),在相同条件下分别测定其 RubisCO 的动力学特性,同质异核水稻间的 RubisCO 的 K_m(O_2)(K。)差异显著,V_(m z)(O_2)(V。)/K。值变化也较大,V_(m z)(CO_2)(V_c)、K_c、V_oV_oK_o/V_oK_o差异不显著。细胞质不同,细胞核相同时,RubisCO 的 K。和 V_cK_o/V_oK_c 值差异显著,K_c 和 V_o 值也变化较大。具有异源细胞质的 RubisCO 比相应的同源 RubisCO 有较高的 RubisC 活性和羧化/加氧比。核质组合对水稻 RubisCO 动力学特性的影响程度依具体核、质组合的不同而异。
The same heterologous nuclear and heterogeneous nuclear rice (Oryzo satiue L.) was obtained by genetic means, and the same large subunit (LS) was extracted, while the different subunits (SS) and LS were different, but the same SS -1, 5-bisphosphate carboxylase / oxygenase (RubisCO) under the same conditions were measured RubisCO dynamic characteristics of RubisCO between homozygous rice K_m (O 2) (K.) significant difference , V_ (mz) (O_2) (V.) / K. V_ (m_ 2) (V_c), K_c, V_oV_oK_o / V_oK_o are also not significantly different. Different cytoplasm, nucleus when the same, RubisCO’s K. And V_cK_o / V_oK_c values significantly different, K_c and V_o values also vary greatly. RubisCO with heterologous cytoplasm has higher RubisC activity and carboxylation / oxygenation ratio than the corresponding cognate RubisCO. The degree of influence of the combination of nuclear and cytoplasm on the kinetic characteristics of rice RubisCO varies with the specific nuclear and matter assemblages.