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目的:探讨神经元活化在癫发生、发展中的作用及托吡酯对其的影响。方法:采用戊四氮制备慢性癫模型,利用托吡酯干扰,选取不同时间点,观察大鼠行为学变化及神经细胞黏附分子在海马回的表达(免疫组织化学染色)。结果:托吡酯组点燃率在27d明显低于模型组;模型组及托吡酯组神经细胞黏附分子表达增加,并随时间延长明显;而不同时间点该表达的增加程度,托吡酯组均低于模型组。结论:神经细胞黏附分子表达的增加,说明出现了神经元活化及脑可塑性变化,而托吡酯明显地抑制了该表达的增加。
Objective: To investigate the role of neuronal activation in the development and progression of epilepsy and the effects of topiramate on it. Methods: Chronic epileptic model was established by using pentylenetetrazole. Topiramate interference was used to observe the changes of behavior and the expression of neural cell adhesion molecules in the hippocampus (immunohistochemical staining) at different time points. Results: The firing rate of topiramate group was significantly lower than that of the model group at 27th day. The expression of adhesion molecules in model group and topiramate group increased significantly with time prolonging. At different time points, the increase of this expression was lower in topiramate group than in model group. CONCLUSIONS: The increased expression of neural cell adhesion molecules indicates neuronal activation and changes in brain plasticity, while topiramate significantly inhibits this increase.