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目的(1)研究过氧化物酶体增殖因子活化受体-γ(PPAR-γ)在慢性非细菌性前列腺炎中的表达,探讨其意义。(2)能否通过其配体噻唑烷二酮类药物(吡格列酮)的激活防治前列腺炎及其可能的机制。方法将45只SD大鼠随机分为正常对照组、慢性非细菌性前列腺炎模型组和慢性非细菌性前列腺炎+吡格列酮治疗组,每组15只。采用RT-PCR对各组PPAR-γ表达情况进行定量分析,以及免疫组化法检测各组NF-κB,IL-1β和TNF-α的表达变化,衡量吡格列酮对慢性非细菌性前列腺炎的防治作用。结果(1)PPAR-γ在正常SD大鼠中几乎没有表达,而在前列腺炎模型组中表达明显增强。(2)吡格列酮能够有效减轻前列腺炎症的发展,并使NF-κB,IL-1β和TNF-α表达下降。结论PPAR-γ可能参与了前列腺炎的发病过程,且其激动剂能够有效减轻炎症程度,可为慢性前列腺炎的治疗提供新的思路。
Objective (1) To investigate the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) in chronic nonbacterial prostatitis and its significance. (2) whether the activation of thiazolidinediones (pioglitazone) can prevent and treat prostatitis and its possible mechanism. Methods Forty-five SD rats were randomly divided into normal control group, chronic non-bacterial prostatitis model group and chronic non-bacterial prostatitis + pioglitazone group, with 15 rats in each group. The expression of PPAR-γ in each group was quantitatively analyzed by RT-PCR and the expressions of NF-κB, IL-1β and TNF-α in each group were detected by immunohistochemistry. The preventive and curative effect of pioglitazone on chronic nonbacterial prostatitis effect. Results (1) PPAR-γ was almost not expressed in normal SD rats, but significantly increased in prostatitis model group. (2) Pioglitazone can effectively reduce the development of prostatitis, and decrease the expression of NF-κB, IL-1β and TNF-α. Conclusion PPAR-γ may be involved in the pathogenesis of prostatitis, and its agonists can effectively reduce the degree of inflammation, which may provide a new idea for the treatment of chronic prostatitis.