论文部分内容阅读
目的探讨慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者痰和血清中白介素-6(interleukin-6,IL-6)的浓度变化及其与气流受限之间的关系。方法采集54例COPD患者和10例正常健康对照者的痰标本及同期血标本,并做肺功能检查,用双抗体夹心ELISA法测定痰和血清中IL-6浓度,分析它们与气流阻塞相关指标第一秒用力呼气容积与预计值百分比(FEV1% pre)、第一秒用力呼气容积占用力肺活量百分比(FEV1/FVC %)、肺残气量(RV)的相关性。结果COPD 各极患者痰中IL-6浓度均高于正常对照组(P<0.05或P<0.01);COPD患者血清IL-6浓度仅在Ⅱb级、Ⅲ级时高于正常对照组(P<0.05)。COPD患者痰中IL-6浓度明显高于自身血清中IL-6浓度(P<0.01),且与FEV1% pre 负相关(r=-0.752,P<0.01),与FEV1/FVC % 负相关(r=-0.693,P<0.01),与RV正相关(r=-0.558,P<0.01);而血清IL-6 浓度与FEV1% pre、FEV1/FVC % 亦存在负相关(r=-0.561,P <0.01;r=-0.473,P <0.01),与RV正相关(r=0.342,P<0.01)。结论COPD患者呼吸道局部IL-6浓度高于外周血IL-6浓度;痰IL-6可更好地反映COPD患者支气管肺泡局部的病理生理变化及气流阻塞的程度;IL-6可能参与了COPD的慢性气道炎症过程及气道重塑过程。
Objective To investigate the changes of sputum and serum interleukin-6 (IL-6) levels in patients with chronic obstructive pulmonary disease (COPD) and their relationship with airflow limitation. Methods Fifty-four COPD patients and 10 healthy controls were included in this study. The sputum samples and the blood samples of the same period were collected for pulmonary function tests. The concentrations of IL-6 in sputum and serum were determined by double-antibody sandwich ELISA. The first second forced expiratory volume and predicted percentage (FEV1% pre), first second forced expiratory volume occupancy of vital capacity (FEV1 / FVC%), pulmonary residual volume (RV) correlation. Results The levels of IL-6 in sputum of patients with COPD were significantly higher than those of normal controls (P <0.05 or P <0.01). The levels of IL-6 in patients with COPD were higher than those in patients with normal controls (P < 0.05). The concentration of IL-6 in sputum of patients with COPD was significantly higher than that of IL-6 in serum (P <0.01), and negatively correlated with FEV1% pre (r = -0.752, P <0.01) (r = -0.693, P <0.01), and positively correlated with RV (r = -0.558, P <0.01). There was also a negative correlation between serum IL-6 concentration and FEV1% pre and FEV1 / P <0.01; r = -0.473, P <0.01), positively correlated with RV (r = 0.342, P <0.01). Conclusions IL-6 in respiratory tract is higher than that in peripheral blood in patients with COPD. Sputum IL-6 can better reflect the pathophysiological changes of bronchial alveoli and the extent of airflow obstruction in COPD patients. IL-6 may be involved in COPD Chronic airway inflammation and airway remodeling.