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在细胞培养液中加入5-溴脱氧尿嘧啶核苷(简称BrdU)后,BruU可以取代细胞新合成的DNA链上的胸腺嘧啶核苷的位置。在含BrdU的培养液中,经历两个细胞分裂周期的细胞,在Giemsa染色后,可以看到染色体上的两条姊妹染色单体,一条浅染,一条深染,浅染的单体表示其DNA双链上只有一股BraU。这种一深一浅的两条染色单体称为姊妹染色单体分化现象(简称SCD)。如果在细胞分裂中,两条姊妹单体曾经发生过交换(简称SCE)的话,则SCE很容易被观察到,计数较准确,方法较简单,比同位
After addition of 5-bromodeoxyuridine (BrdU) to the cell culture medium, BruU can replace the thymidine position on the newly synthesized DNA strand of the cell. In BrdU-containing broth, cells that underwent two cycles of cell division showed two sister chromatids on the chromosome after Giemsa staining. A lightly stained, one stained, lightly stained cell indicates that There is only one strand of BraU on the DNA double strand. The darker, darker two chromatids are called sister chromatid degeneration (SCD). If in the cell division, the two sisters have been exchanged (SCE), then the SCE can be easily observed, the count is more accurate, the method is simpler, than the same bit