论文部分内容阅读
目的了解和掌握青岛地区无偿献血人群HIV感染情况,比较分析3种检测方法的结果,为加强血液管理提供决策依据。方法将本中心2013年6月14日-2014年6月14日采集的血液样本100 885人份进行血清学检测,其中99 637例ALT正常,TP抗体阴性的献血者样本进行了NAT,抗-HIV初筛阳性样本送往市疾病控制中心做确证实验,对3种方法的检测结果进行回顾性分析。结果抗-HIV反应性59例(阳性率为0.06%),其中抗-HIV和核酸同时反应性16例(阳性率为0.016%),43例抗-HIV单项反应性,未检出HIV核酸单项反应性样本,59例经确证阳性18例(感染率为17.84/10万),1例不确定,其中16例抗-HIV和核酸同时反应性样本经确证全部HIV阳性,43例抗-HIV单项反应性确证阳性2例,1例不确定。在16例血清学反应性(S/CO值大于15)、NAT阳性样本中,核酸检测的阳性预测值为100%。流行病学调查发现18例确证阳性献血者全部为男性,未婚11例(61.11%),已婚6例(33.33%),1例不祥;首次献血者8例(44.44%),重复献血者10例(55.56%)。结论 NAT的假阳性率远远低于血清学检测方法,针对HIV感染检测准确度高。为了保证血液质量和输血安全,除了加强实验室管理,提高HIV检测水平,我们应积极普及无偿献血知识和艾滋病相关知识的宣传教育,加强未婚男性青年的艾滋病防治工作。
Objective To understand and master HIV infection in unpaid blood donors in Qingdao and to compare and analyze the results of three test methods to provide decision-making basis for strengthening blood management. Methods A total of 100 885 blood samples collected from our center from June 14, 2013 to June 14, 2014 were tested for serology. Among them, 99 637 samples of normal and TP antibody-negative donors were tested for NAT, HIV positive screening samples sent to the city center for disease control to confirm the test results of the three methods were retrospectively analyzed. Results The anti-HIV activity was 59 (positive rate 0.06%). Among them, 16 cases were positive for anti-HIV and nucleic acid (positive rate was 0.016%), 43 cases were anti-HIV single- Reactive samples, of which 59 were confirmed positive in 18 cases (infection rate of 17.84 / 100000), 1 case of uncertainty, of which 16 cases of anti-HIV and nucleic acid simultaneously confirmed positive samples of all HIV-positive, 43 anti-HIV single Positive reactivity confirmed in 2 cases, 1 case not sure. The positive predictive value for nucleic acid testing was 100% in 16 serologically reactive (S / CO values greater than 15) and NAT positive samples. Epidemiological survey found that 18 cases of positive blood donors were all male, 11 cases were unmarried (61.11%), 6 cases were married (33.33%), 1 case was ominous. The first donors were 8 cases (44.44%), repeat donors 10 Cases (55.56%). Conclusion The false positive rate of NAT is far lower than that of serological test, and the detection accuracy of HIV infection is high. In order to ensure blood quality and blood transfusion safety, in addition to strengthening laboratory management and improving HIV testing level, we should actively promote the education of unpaid blood donation and AIDS-related knowledge and strengthen the AIDS prevention and control of unmarried young men.