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从大田实生苗和无性系苗圃收集两个桉树耐寒种毛皮桉(Eucalyptusmacarthurii)、谷桉(E.smithii),一个耐寒杂交种 MG 25(E.macarthurii×E.grandis)和柳桉(E.saligna)的有节外殖体进行离体繁殖。小苗最初生长在含有0.1毫克/升6-苄氨基嘌呤(BA)的改良 MS 培养基上。含有6-苄氨基嘌呤(0.2毫克/升)和萘乙酸(0.01毫克/升)的改良 MS 培养基对促进芽的增殖最有效。在含有2毫克/升萘乙酸的半固化改良 MS 培养基上诱导发根,已长根的小苗经健化后移植大田。
Two Eucalyptus macarthurii, E. smithii, one cold-tolerant hybrid, MG 25 (E. macarthurii × E. grandis) and E. saligna (Eucalyptus) were collected from field seedlings and clonal nurseries ) Of the euthenium ex vivo breeding. Seedlings were initially grown on modified MS medium containing 0.1 mg / L 6-benzylaminopurine (BA). Modified MS medium containing 6-benzylaminopurine (0.2 mg / L) and NAA (0.01 mg / L) is most effective at promoting bud proliferation. Roots were induced on a semi-cured MS medium containing 2 mg / L naphthaleneacetic acid, and young shoots that had long roots were transplanted to the field after being harvested.