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目的探讨受激活调节正常T细胞表达和分泌因子(RANTES)在胃癌中的表达及其意义。方法应用免疫组织化学、蛋白印迹技术检测胃癌患者(8例)和正常人(8例)胃组织中RANTES蛋白表达;ELISA检测RANTES在胃癌患者和正常人外周血中的浓度。结果免疫组织化学和蛋白印迹技术检测胃癌组织中RANTES蛋白的表达分别为(2.38±0.52)分和117 940.00±1572.15,明显高于正常胃组织的(0.63±0.52)分和4129.25±287.19(P<0.05或P<0.01);胃癌患者外周血RANTES浓度高于正常人[(685.25±296.19)pg/ml vs.(174.88±70.50)pg/ml](P<0.05)。结论 RANTES与胃癌密切相关,有可能成为胃癌治疗的新靶点。
Objective To investigate the expression and significance of RANTES regulated by activated normal T cell in gastric cancer. Methods The expressions of RANTES protein in gastric cancer tissues (8 cases) and normal gastric tissues (8 cases) were detected by immunohistochemistry and Western blotting. The peripheral blood levels of RANTES in gastric cancer patients and normal controls were detected by ELISA. Results The expressions of RANTES protein in gastric cancer tissues were (2.38 ± 0.52) and 117 940.00 ± 1572.15 respectively, which were significantly higher than those in normal gastric tissues (0.63 ± 0.52) and 4129.25 ± 287.19 (P < 0.05 or P <0.01). The concentration of RANTES in peripheral blood of patients with gastric cancer was higher than that of normal people [(685.25 ± 296.19) pg / ml vs. (174.88 ± 70.50) pg / ml] (P <0.05). Conclusions RANTES is closely related to gastric cancer and may be a new target for the treatment of gastric cancer.