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有研究表明,NK样细胞出现于胚胎的植入部位,其生理学功能尚不明确。有人认为这些细胞可阻止滋养层细胞的过度侵入,有利于妊娠,但有时也能损害胚胎导致自发性流产。所以母体蜕膜自细胞的局部免疫调节作用,对成功妊娠必不可少。本研究阐述了兔BF D-12(妊娠12天的囊胚腔液)中的PGE_2对母体NK细胞和LAK细胞细胞毒活性的调节作用。 将动情期定为实验0天。该日使兔交配后注射75 IU的hCG确保排卵。于妊娠第8天取血。以Histopaque 1 077(Sigma)密度梯度离心法分离出外周血淋巴细胞(PBL),加入rlL-2培养至妊娠第12天,收集细胞,即LAK细胞。该日再次取血分离PBL,获得NK细胞。同时取囊胚腔液,收集滋养层
Some studies have shown that NK-like cells in the embryo implantation site, its physiological function is not clear. Some people think that these cells can prevent the trophoblast cells from excessive invasion, is conducive to pregnancy, but sometimes can damage the embryo lead to spontaneous abortion. So the mother decidual cells from the local immune regulation, essential for successful pregnancy. This study describes the regulatory effect of PGE 2 in rabbit BF D-12 (blastocyst fluid of 12-day gestation) on the cytotoxic activity of maternal NK cells and LAK cells. The estrus as experiment 0 days. Rabbits were mated to 75 IU of hCG on that day to ensure ovulation. Blood was taken on the 8th day of pregnancy. Peripheral blood lymphocytes (PBLs) were isolated by density gradient centrifugation with Histopaque 1 077 (Sigma) and cultured in rlL-2 until day 12 of gestation. Cells were collected, ie, LAK cells. Blood was taken again on that day to isolate PBL and obtain NK cells. At the same time get blastocyst fluid, collecting trophoblast