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目的了解胰腺癌细胞DNA倍型及临床意义。方法68例胰腺癌组织标本，行Feulgen染色，应用全自动图像分析仪测定癌细胞DNA含量及倍型。结果本组2倍体／近2倍体（2C）率及异倍体（AN）率分别为26.5％及73．5％。2C肿瘤比AN有较高的手术切除率，较好的预后（P＜0．05），手术时实测2C肿瘤体积小，临床分期早。DNA倍型与病理分级无关（P＞0．05），而与临床分期、肿瘤大小相关（P＜0．01）。结论胰腺癌细胞异倍体率高，临床上表现为恶性程度高，手术切除率低，预后差。
Objective To understand the DNA phenotype of pancreatic cancer cells and its clinical significance. Methods Feltgen staining was performed on 68 cases of pancreatic cancer. DNA content and haplotype of cancer cells were determined by automatic image analyzer. Results The rates of diploid/near-diploid (2C) and aneuploid (AN) in this group were 26.5% and 73.5%, respectively. 2C tumors had a higher surgical resection rate than AN and a better prognosis (P<0.05). The measured 2C tumor volume was small at the time of surgery and the clinical stage was early. DNA ploidy was not associated with pathological grade (P>0.05), but was related to clinical stage and tumor size (P<0.01). Conclusions The aneuploidy rate of pancreatic cancer cells is high, and the clinical manifestations are high degree of malignancy, low surgical resection rate and poor prognosis.