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选用无各型肝炎病毒感染、体质良好的6只食蟹猴、3只恒河猴和3只熊猴,按人感染HCV的主要途径——输血,经静脉输注含HCV的中国献血员血浆,观察临床一般指标,血清ALT,抗HCV各种抗体和HCV-RNA,肝组织普通病理,超微病理。从肝组织中找病毒颗粒及免疫组化查HCV NS3抗原,观察14周,结果9例猕猴出现不同程度的肝炎症状,12例血清ALT升高,2例出现黄疸;5例抗HCVC22 IgG抗体阳性,4例抗HCV E2-24 IgG抗体阳性,10例抗HCV C33c IgG抗体阳性,2例抗HCVNS4-40 IgG抗体阳性,11例抗HCV NS5-A IgG抗体阳性,无1例抗HCVNS5-B IgG抗体阳性;9例HCV-RNA阳性。6例肝活检或肝小部切除作肝组织普通光镜镜检,5例肝细胞气球样变,点状坏死及少许纤维组织增生。另一例相应损害较重,呈碎片坏死,界板破坏,纤维组织增生明显,汇管区炎性细胞浸润,超微病理除普通炎症所具有的特征外,以滑面内质网大量增生扩张为其主要特征,3例从内质网里看到约30nm的类病毒颗粒。免疫组化显示3只动物肝组织内HCVNS3抗原阳性,以膜型为主少量周边型及散在胞浆内分布,因此我们认为已初步获得HCV输血感染的急性丙型肝炎的猕猴模型。
Six healthy cynomolgus monkeys, three rhesus monkeys and three bears monkeys were selected for hepatitis C virus infection. According to the main route of human infection of HCV - transfusion, the plasma of Chinese donors with HCV was transfused intravenously, Clinical general indicators, serum ALT, anti-HCV antibodies and HCV-RNA, common pathological liver tissue, ultra-pathological. From the liver tissue to find the virus particles and immunohistochemical detection of HCV NS3 antigen, observed for 14 weeks, the results of nine cases of rhesus monkeys showed varying degrees of hepatitis symptoms, 12 cases of elevated serum ALT, 2 cases of jaundice; 5 cases of anti-HCVC22 IgG positive , 4 anti-HCV E2-24 IgG antibodies, 10 anti-HCV C33c IgG antibodies, 2 anti-HCV NS4-40 IgG antibodies, 11 anti-HCV NS5-A IgG antibodies and none anti-HCV NS5-B IgG Antibody positive; 9 cases of HCV-RNA positive. 6 cases of liver biopsy or liver resection for liver microscopy, 5 cases of hepatocyte balloon-like changes, punctate necrosis and a small amount of fibrous tissue hyperplasia. Another case of severe damage, was debris necrosis, destruction of the boundary, fibrous tissue proliferation significantly, the portal area inflammatory cell infiltration, ultra-microscopic pathology in addition to common features of inflammation, with a large number of glomerulus endoplasmic reticulum proliferation expansion The main feature, 3 cases from the endoplasmic reticulum to see about 30nm of the virus-like particles. Immunohistochemistry showed that HCVNS3 antigen was positive in the liver tissue of three animals, which was mainly distributed in the cytoplasm with few membranous peripheries and scattered in the cytoplasm. Therefore, we think that the HCV infection in acute rhesus monkeys infected with HCV is preliminarily obtained.