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为了观察1 -甲基- 4 -苯基- 1, 2, 3, 6 四氢吡啶(MPTP)对C57BL/6小鼠空间学习记忆能力和脑内强啡肽(DYN)表达强度的影响,本研究给予小鼠皮下注射MPTP20mg/kg,连续8d,制备Parkinson病(PD)模型。与对照组相比,MPTP处理小鼠爬竿(P<0. 01)和游泳(P<0. 01)分数明显降低。Morris水迷宫实验结果显示:MPTP处理小鼠寻找平台潜伏期明显延长(P<0.01),在目标及对侧象限游泳时间所占百分比分别明显降低(P<0. 01)和增加(P<0. 01)。免疫细胞化学结果表明中脑黑质致密部的酪氨酸羟化酶(TH)免疫阳性神经元数目明显减少(P<0. 01)。同时在前额叶皮层(P<0. 01 )、背侧海马的CA1区(P<0. 05)和齿状回苔状纤维通路(P<0. 05)、背侧尾核(P<0. 01)的DYN免疫反应活性明显增强。本实验结果表明:用MPTP处理诱发的PD小鼠模型伴有空间学习和记忆能力的下降;并提示前额叶皮层、背侧海马的CA1区、齿状回苔状纤维通路和背侧尾核内DYN表达的增多可能与MPTP小鼠空间学习和记忆能力下降有关。
In order to observe the effect of 1 - methyl - 4 - phenyl - 1, 2, 3, 6 tetrahydropyridine (MPTP) on spatial learning and memory ability and dynorphin (DYN) expression in C57BL / 6 mice, Study mice were given subcutaneous injection of 20mg / kg MPTP, continuous 8d, Parkinson’s disease (PD) model was prepared. Compared with the control group, the MPTP-treated mouse climb pole (P <0.01) and swimming (P <0.01) decreased significantly. The results of Morris water maze test showed that the latency of MPTP-treated mice in searching for platform was significantly longer (P <0.01), and the percentage of swimming time in target and contralateral quadrant was significantly lower (P <0.01) and increased (P <0. 01). Immunocytochemistry showed that the number of tyrosine hydroxylase (TH) immunoreactive neurons in substantia nigra pars compacta decreased significantly (P <0.01). At the same time, in the prefrontal cortex (P <0.01), dorsal hippocampus CA1 (P <0.05) and dentate moss fiber pathways (P <0.05), dorsal caudate nucleus (P <0 01) DYN immunoreactivity was significantly enhanced. The experimental results show that MPTP-induced PD mice model with spatial learning and memory loss decreased; and prompted the prefrontal cortex, the dorsal hippocampus CA1 area, dentate mossy fiber pathways and dorsal caudate nucleus Increased expression of DYN may be associated with decreased spatial learning and memory in MPTP mice.