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目的研究乳腺癌不同分子亚型中癌干细胞量的差别。方法根据分子亚型将乳腺癌组织标本分为A(乳腺腔内A型),B(乳腺腔内B型),C(HER-2过表达型),D(基底样型),E(正常细胞样型)5组,通过集落细胞法计数各组癌干细胞球,分析乳腺癌干细胞量与乳腺癌不同分子亚型的关系。结果 A,B两组的癌干细胞球数分别为(1.1±0.2)/1 000细胞和(1.3±0.1)/1 000细胞,组间差异无统计学意义(P>0.05);C组的癌干细胞球数为(8.6±1.0)/1 000细胞,明显多于A,B组(均P<0.05);D,E两组的癌干细胞球数分别为(22.4±1.2)/1 000细胞和(17.7±2.0)/1 000细胞,两组间差异无统计学意义(P>0.05),但均明显多于前3组(均P<0.05)。结论不同分子亚型乳腺癌组织中含有不同量的癌干细胞,对预测乳腺癌预后可能有一定意义。
Objective To study the difference of cancer stem cells in different molecular subtypes of breast cancer. Methods According to the molecular subtypes, the breast cancer tissues were divided into A (breast cavity type A), B (breast cavity type B), C (HER-2 overexpression), D (basal type), E Cell type), and count the number of stem cells of each group by colony cell method to analyze the relationship between the amount of breast cancer stem cells and different molecular subtypes of breast cancer. Results The numbers of cancer stem cells in groups A and B were (1.1 ± 0.2) / 1 000 cells and (1.3 ± 0.1) / 1 000 cells, respectively. There was no significant difference between the two groups (P> 0.05) The numbers of spheres in stem cells were (8.6 ± 1.0) / 1 000 cells, which were significantly more than those in groups A and B (all P <0.05). The numbers of spheres of cancer stem cells in groups D and E were (22.4 ± 1.2) (17.7 ± 2.0) / 1 000 cells. There was no significant difference between the two groups (P> 0.05), but both of them were significantly more than the former three groups (all P <0.05). Conclusion Different molecular subtypes of breast cancer tissues contain different amounts of cancer stem cells, which may have some significance in predicting the prognosis of breast cancer.