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目的:探讨腹腔手术对老龄大鼠认知功能的影响,建立老龄术后认知功能障碍大鼠模型。方法:18月龄雄性SD大鼠45只,体重500-650g,随机分为3组:对照组(C组,n=15)、麻醉组(A组,n=15)和手术组(S组,n=15)。采用脾切除+阑尾切除+腹腔探查术建立老龄大鼠术后认知功能障碍模型。C组腹腔注射生理盐水3ml;A组腹腔注射1%戊巴比妥钠注射液50mg/kg(稀释至3ml)麻醉,不行手术;S组腹腔注射与A组等量的麻醉药物后行脾切除+阑尾切除+腹腔探查术。各组随机分为3个亚组(n=5),于麻醉或手术结束后第1,3,7天采用Y迷宫实验和旷场分析实验测试大鼠认知功能(即C1、C3、C7组,A1、A3、A7组,S1、S3、S7组)。结果:各组大鼠术前Y迷宫测试指标差异无统计学意义(P>0.05)。与C组比较,A组大鼠术后各时点Y迷宫测试指标正确反应次数(RN)及全天总反应时间(TRT)差异无统计学意义(P>0.05);术后第1,3天,与C、A组相比,S组大鼠RN减少,TRT延长(P<0.01);术后第7天,与C、A组相比,S组大鼠RN及TRT差异无统计学意义(P>0.05)。与C组比较,A组大鼠术后各时点旷场分析指标差异无统计学意义(P>0.05)。术后第1,3天,与C、A组相比,S组大鼠中央格时间延长,跨格及站立次数减少(P<0.01,P<0.05);术后第7天,与C、A组相比,S组大鼠中央格时间、跨格次数、站立次数差异无统计学意义(P>0.05)。结论:脾切除+阑尾切除+腹腔探查术可导致老龄大鼠术后认知功能障碍,该模型可作为研究手术应激导致术后认知功能障碍的动物模型。
Objective: To investigate the effect of abdominal surgery on cognitive function in aged rats and to establish a cognitive impairment model in aged rats. Methods: 45 male 18-month-old male SD rats weighing 500-650 g were randomly divided into three groups: control group (group C, n = 15), anesthesia group (group A, n = 15) and operation group , n = 15). Splenectomy + appendectomy + celiac exploration was used to establish the postoperative cognitive impairment model in aged rats. Group C was intraperitoneally injected with normal saline 3ml; group A was injected intraperitoneally with 1% pentobarbital sodium injection 50mg / kg (diluted to 3ml) for anesthesia without surgery; group S was intraperitoneally injected with the same dose of anesthesia as group A Appendectomy + abdominal exploration. The rats in each group were randomly divided into 3 subgroups (n = 5). After anesthesia or on the 1st, 3rd, 7th day after operation, Y maze test and open-field test were used to test the cognitive function of rats Group, A1, A3, A7 group, S1, S3, S7 group). Results: There was no significant difference in preoperative Y-maze test between the two groups (P> 0.05). Compared with group C, there was no significant difference in the number of correct reaction times (RN) and total daytime response time (TRT) between Y group and control group (P> 0.05) Day, compared with group C and group A, RN of TR group was decreased and TRT was prolonged (P <0.01). On the 7th day after surgery, RN and TRT of rats in S group were not statistically different from those in C and A groups Significance (P> 0.05). Compared with group C, there was no significant difference in open field analysis between groups A and B (P> 0.05). On the 1st and 3rd day after operation, compared with the C and A groups, the central grid time, the number of cross-grid and standing decreased in S group (P <0.01, P <0.05) Compared with group A, there was no significant difference in central grid time, number of interclass and number of standing in group S (P> 0.05). Conclusions: Splenectomy + appendectomy + celiac exploration can lead to postoperative cognitive impairment in aged rats. This model can be used as an animal model to study postoperative cognitive dysfunction caused by surgical stress.