Size Depending Separation of HAP:Eu Nanoparticles in Dispersed Sol

• 作 者: I W Bauer HAN Yingchao LI Ship
• 机 构: Biomedical Materials and Engineering Research Center
• 出 处: 武汉理工大学学报英
• 发表日期: 2004年期
• 关键词: nanoparticles size depending separation centrifuge hydroxyapatite europium

The particle size has a strong impact on the interactions between nanoparticles and cells. However, the synthesis process of nanoparticles limits the range of achievable average particle sizes. When biocompatible hydroxyapatite nanoparticles (HAP) are doped with the luminescent rare earth element Europium (Eu), the particle size becomes larger compared to pure HAP. Hence, a particle size reduction is necessary to achieve similar experimental conditions when substituting pure HAP with luminescent HAP: Eu nanoparticles to investigate particlecell-interactions in cell culture experiments. While the sedimentation process of particles in liquids and gels has been well described in literature, the separation of particles in dispersed colloids has not been studied, yet. In this study, the size depending separation and particle size reduction of a homogeneous dispersed nanoparticle sol by gravity and centrifugation were investigated. As the results showed, shorter time of centrifugation at higher speed can reduce the average particle size compared to the decline of the particle concentration in the upper sol layer most efficiently. This centrifugation method has some similarity to the overspeeding technique which is commonly used to lower the transient time to reach the equilibrium of sedimentation.