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运用链特异性逆转录聚合酶链式反应法(RT-PCR)建立了一种快速、灵敏、特异的脊髓灰质炎灭活疫苗(IPV)的灭活验证体系.以Sabin株脊髓灰质炎病毒悬液作为阳性对照,对脊髓灰质炎灭活疫苗进行检测.结果表明,链特异性RT-PCR法对具有活性的脊髓灰质炎病毒检测为阳性,而对脊髓灰质炎灭活疫苗的检测结果为阴性.链特异性RT-PCR法可作为一种简便、快速、灵敏的脊髓灰质炎灭活疫苗灭活验证方法,大大缩短了检测周期,在脊髓灰质炎灭活疫苗灭活验证的常规检测中具有较好的实际应用价值.
A rapid, sensitive and specific inactivation verification system of inactivated poliomyelitis vaccine (IPV) was established by using strand-specific reverse transcriptase-polymerase chain reaction (RT-PCR) Liquid as a positive control, the polio inactivated vaccine was tested.The results showed that chain-specific RT-PCR method for the detection of active poliovirus positive, and the results of the test for inactivated poliomyelitis vaccine was negative Chain-specific RT-PCR method can be used as a simple, rapid and sensitive method to inactivate poliovirus inactivated vaccine, greatly shortening the detection cycle, and has the advantages of routine test in inactivation of polio inactivated vaccine Good practical value.