论文部分内容阅读
目的对体外受精-胚胎移植(IVF-ET)中未受精卵母细胞采用卵细胞胞浆内单精子注射(ICSI)作为补救受精方法后结局进行评价。方法回顾分析采用上述ICSI补救方法的11个周期病人,均以女方不孕因素为主进行IVF-ET治疗,在行常规受精18h后观察受精失败,即行ICSI再受精(Late ICSI),胚胎形成后行胚胎移植术,术后21d明确有无临床妊娠。结果11例病人共取出135只卵子,其中MII期107只,MI期23只,GV期5只,常规受精后只有2.80%受精(3/107)。随即对103只未受精卵行Late ICSI,术后受精率达到79.61%(82/103),卵裂率74.39(61/82),共移植25只胚胎,无一例临床妊娠。结论ICSI技术作为补救受精的方法能增加受精率,卵裂率,但不增加妊娠率;常规IVF-ET受精率低与男方精子活力低的关系有待进一步探讨。
Objective To evaluate the outcome of intracytoplasmic sperm injection (ICSI) of unfertilized oocytes in vitro fertilization-embryo transfer (IVF-ET) as a remedial fertilization method. Methods Retrospective analysis of the 11 cycles of ICSI recuperation patients were based on female infertility IVF-ET treatment, routine fertilization after 18h failed to observe fertilization, that is, ICSI fertilization (Late ICSI), after embryogenesis Line embryo transfer surgery, 21d postoperative clear whether the clinical pregnancy. Results A total of 135 eggs were obtained in 11 patients, including 107 in MII, 23 in MI, and 5 in GV. Only 2.80% fertilized (3/107) after conventional fertilization. Immediately afterwards, 103 ICSIs were fertilized with Late ICSI. The fertilization rate reached 79.61% (82/103) and the cleavage rate was 74.39 (61/82). A total of 25 embryos were transplanted without any clinical pregnancy. Conclusion ICSI technique as a fertilization method can increase fertilization rate and cleavage rate, but does not increase pregnancy rate. The relationship between low IVF-ET fertility rate and male sperm motility is yet to be further explored.