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目的 通过对颈淋巴结中树突状细胞 (ｄｅｎｄｒｉｔｉｃｃｅｌｌｓ,ＤＣ)浸润密度进行定量分析 ,探讨ＤＣ在喉癌患者抗肿瘤免疫反应中的作用。方法 47例行选择性颈淋巴结清扫术的喉癌患者的 15 7枚颈淋巴结石蜡标本被重新制备 ,用抗Ｓ 10 0蛋白单克隆抗体免疫组化ＳＡＢＣ法
Objective To quantitatively analyze the density of dendritic cells (DCs) infiltration in cervical lymph nodes to explore the role of DCs in anti-tumor immune response in patients with laryngeal cancer. Methods Fifty-seven cervical lymph node paraffin specimens of laryngeal cancer patients undergoing selective cervical lymph node dissection were re-prepared, and immunohistochemical SABC method with anti-S 10 protein monoclonal antibody