抗子宫内膜抗体和子宫内膜异位症

• 作 者: 李伟毅 陆勇娟 朱云凤 程丽萍 席晔斌 陈广洁 周文达
• 机 构: 上海第二医科大学上海市免疫学研究所,上海第二医科大学附属瑞金医院妇产科,上海第二医科大学医学检验实验室
• 出 处: 中国免疫学杂志
• 发表日期: 1999年06期
• 关键词: 子宫内膜异位症 子宫内膜抗原 抗子宫内膜抗体

目的：研究抗子宫内膜抗体（ＥＭＡｂ）与子宫内膜异位症（内异症）的关系。方法：改良法提取、纯化正常人及内异症患者的子宫内膜抗原（ＥＭＡｇ），分析其氨基酸组成，并用ＳＤＳＰＡＧＥ及Ｗｅｓｔｅｒｎｂｌｏｔ分析两者蛋白质组成及抗原特异性；建立ＥＬＩＳＡ方法并测定、分析正常人及内异症患者血清ＥＭＡｂ。结果：正常人ＥＭＡｇ有２条蛋白质条带（４８０ｋＤ和大于９７．４ｋＤ）；内异症患者则有３条蛋白质条带（４６０、５４０和大于９７．４ｋＤ），前者仅１条蛋白质条带（大于９７．４ｋＤ），后者则３条蛋白质条带可与ＥＭＡｂ特异反应，且两者在氨基酸组成上亦有差异；内异症患者血清ＥＭＡｂ阳性率明显高于正常人及非内异症（其它妇科疾病）患者。结论：ＥＭＡｂ与内异症密切相关。

Objective: To study the relationship between anti-endometrial antibody (EMAb) and endometriosis (endometriosis). Methods: The endometrial antigen (EMAg) of normal and endometriosis patients was extracted and purified by the improved method. The amino acid composition was analyzed. The protein composition and antigen specificity were analyzed by SDSPAGE and Western blotting. ELISA method was established and assayed. Analysis of normal and endometriosis serum EMAb. Results: There were two protein bands (480kD and more than 97.4kD) in normal human EMAg, three protein bands (460,540 and more than 97.4kD) in endometriosis patients, the former only 1 protein band (greater than 97.4kD), while the latter three protein bands with EMAb-specific reaction, and both in the amino acid composition is also different; endometriosis serum EMAb positive rate was significantly higher than normal And non-endometriosis (other gynecological diseases) patients. Conclusion: EMAb is closely related to endometriosis.