论文部分内容阅读
目的探讨可溶性CD40配体(sCD40L)在大动脉粥样硬化性卒中(LAA)患者的表达及意义。方法用ELISA法连续测定29例LAA患者(A组)和11例健康体检者(B组)血浆sCD40L、血小板膜蛋白140(GMP-140)和白细胞介素6(IL-6)含量。结果与B组比较,A组血小板GMP-140含量增高[(43.78±16.61)ng/ml vs.(9.42±2.96)ng/ml](P<0.01),sCD40L和IL-6亦明显增高[(8.36±4.30)ng/ml vs.(3.52±1.69)ng/ml和(55.59±18.20)pg/ml vs.(30.33±7.45)pg/ml](P<0.01)。A组患者sCD40L与GMP-140水平、sCD40L与IL-6水平、GMP-140与IL-6水平均呈正相关。结论 LAA患者血循环中sCD40L含量升高,提示血小板源性CD40L是炎症、动脉粥样硬化斑块不稳定和血栓形成的一个重要介质。循环中sCD40L升高可作为血小板活化、不稳定动脉粥样斑块发展为缺血性卒中的标志物。
Objective To investigate the expression and significance of soluble CD40 ligand (sCD40L) in patients with large atherosclerotic stroke (LAA). Methods Serum levels of sCD40L, platelet membrane protein 140 (GMP-140) and interleukin 6 (IL-6) in 29 LAA patients (group A) and 11 healthy subjects (group B) were determined by ELISA. Results Compared with group B, the levels of platelet GMP-140 in group A were significantly increased (P <0.01) 8.36 ± 4.30 ng / ml vs. 3.52 ± 1.69 ng / ml and 55.59 ± 18.20 pg / ml vs. 30.33 ± 7.45 pg / ml], respectively (P <0.01). A group of patients sCD40L and GMP-140 levels, sCD40L and IL-6 levels, GMP-140 and IL-6 levels were positively correlated. Conclusions The increase of sCD40L in blood circulation of LAA patients suggests that platelet-derived CD40L is an important mediator of inflammation, atherosclerotic plaque instability and thrombosis. Circulation sCD40L increased as platelet activation, unstable atherosclerotic plaque developed as a marker of ischemic stroke.