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目的 研究PGC 1基因Gly482Ser变异与汉族人群 2型糖尿病的关系。 方法 用聚合酶链反应 限制性片段长度多态性分析方法检测 15 4例 2型糖尿病患者 (糖尿病组 )及 13 0例糖耐量正常者 (对照组 )PGC 1基因Gly482Ser变异的基因型 ,全自动生化分析仪检测甘油三脂、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇 ,空腹血糖等。结果 (1)糖尿病组等位基因频率与对照组比较有显著差异 (P =0 .0 0 1)。 (2 )携带Ser等位基因发生 2型糖尿病的相对风险的比数比为 1.73 1(95 %的可信区间为 1.2 40～ 2 .417)。 (3 )方差分析发现PGC 1基因Gly482Ser变异对C肽、脂蛋白a、载脂蛋白B有影响 (P为 0 .0 48、0 .0 0 7、0 .0 45 )。结论 PGC 1基因Gly482Ser变异与汉族人群 2型糖尿病的发生相关
Objective To study the relationship between Gly482Ser mutation in PGC1 gene and type 2 diabetes in Han population. Methods The genotypes of Gly482Ser mutation in PGC1 gene were detected in 15 4 type 2 diabetic patients (diabetic group) and 130 normal glucose tolerance patients (control group) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Biochemical analyzer triglyceride, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, fasting blood glucose and so on. Results (1) The frequency of allele in diabetic group was significantly different from that in control group (P = 0.010). (2) The odds ratio for the relative risk of type 2 diabetes with the Ser allele was 1.73 1 (95% confidence interval, 1.2 40-2.417). (3) ANOVA analysis showed that Gly482Ser mutation of PGC1 gene had influence on C peptide, lipoprotein a and apolipoprotein B (P = 0.048, 0.07,0.045). Conclusion The Gly482Ser mutation of PGC1 gene is associated with the occurrence of type 2 diabetes in Han population