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目的探讨血管内皮细胞生长因子-C(VEGF-C)与血管内皮细胞生长因子受体VEGFR-3(Flt4),在前列腺癌中的表达以及结核菌L型感染率及临床意义。方法应用免疫组化和原位杂交和抗酸染色等方法检测65例前列腺癌(carcinoma of prostate,PCa)和30例良性前列腺增生(benign prostatic hyperplasia,BPH)中的VEGF-C、Fit4蛋白及mRNA的表达,以及结核菌L型的检出率。并对前列腺肿瘤主要临床资料和病理分级参数进行比较,用χ2检验进行统计学处理。结果VEGF-C、Flt4蛋白及mRNA阳性表达率和结核菌L型检出率,前列腺癌明显高于前列腺增生(P<0.001~0.05)。VEGF-C、Flt4蛋白及mRNA阳性表达率和结核菌L型检出率与前列腺癌的临床分期、病理分级有明显差异(P<0.05)。淋巴结转移组中VEGF-C、Flt4蛋白及mRNA的阳性表达率明显高于非转移组(P<0.01)。结核菌L型检出率淋巴结转移组明显高于非转移组(P<0.05)。结论VEGF-C、Flt4的蛋白及mRNA在前列腺肿瘤中不同程度异常表达以及结核菌L型检出率与肿瘤的临床分期、病理分级和转移呈正相关,提示这2种基因均可作为判断前列腺癌生物学行为及患者预后参考指标;结核菌L型感染可能有协同致瘤作用,与前列腺癌的发生发展可能有一定关系。因此研究VEGF-C、Flt4的阳性表达和结核菌L型感染与前列腺癌的关系,具有重要的临床应用价值。
Objective To investigate the expression of vascular endothelial cell growth factor-C (VEGF-C) and vascular endothelial growth factor receptor VEGFR-3 (Flt4) in prostate cancer and its clinical significance. Methods The expressions of VEGF-C, Fit4 protein and mRNA in 65 cases of prostate cancer (PCa) and 30 benign prostatic hyperplasia (BPH) were detected by immunohistochemistry, in situ hybridization and acid-fast staining Of the expression, as well as the detection rate of TB L-type. The main clinical data of prostate tumors and pathological grade parameters were compared, using χ2 test for statistical analysis. Results The positive rate of VEGF-C, Flt4 protein and mRNA expression and the detection rate of L-form of tuberculosis were significantly higher in prostate cancer than in benign prostatic hyperplasia (P <0.001 ~ 0.05). The positive rate of VEGF-C, Flt4 protein and mRNA and the detection rate of L-form of tuberculosis were significantly different from those of prostate cancer (P <0.05). The positive rates of VEGF-C, Flt4 protein and mRNA in lymph node metastasis group were significantly higher than those in non-metastasis group (P <0.01). Mycobacterium tuberculosis L-type positive rate of lymph node metastasis was significantly higher than non-metastasis group (P <0.05). Conclusions The abnormal expression of VEGF-C and Flt4 protein and mRNA in prostate tumor and the positive detection rate of L-form of tuberculosis are positively correlated with the clinical stage, pathological grade and metastasis of tumor, suggesting that these two genes can be used as a marker for the diagnosis of prostate cancer Biological behavior and prognosis of patients with reference markers; Mycobacterium L-type infection may have collaborative tumorigenesis, and the occurrence and development of prostate cancer may have a certain relationship. Therefore, the study of the positive expression of VEGF-C, Flt4 and the relationship between tuberculosis L-type infection and prostate cancer have important clinical value.