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为抗除草剂转基因水稻食用安全性评价中的致敏性评估提供参考,对抗除草剂转基因稻米蛋白的消化稳定性和热稳定性进行分析。体外模拟胃肠液消化和热加工处理抗除草剂转基因水稻Bar68-1稻米蛋白、非转基因亲本对照D68稻米蛋白、阴性对照酪蛋白、阳性对照大豆胰蛋白酶抑制剂和牛血清蛋白,通过SDS-PAGE凝胶电泳观察它们在各时间点的降解情况。研究结果显示:消化稳定性试验中,转基因稻米蛋白和非转基因稻米蛋白在2~30 min内全部消化,可判断它们在模拟胃、肠液中可消化;热稳定性试验中,转基因稻米蛋白和非转基因稻米蛋白一样,在一定温度下(60、75、90、100℃)加热一定时间(15、30、60、120 min)后仍具有较好的热稳定性。结果表明,转基因抗除草剂水稻Bar68-1具有较好的热稳定性,在模拟胃液和模拟肠液中均可消化,不具备过敏原抗消化性的特征。
This study provides a reference for evaluating the allergenicity of herbicide-adapted transgenic rice in food safety evaluation and analyzes the digestibility and thermostability of the herbicide-modified transgenic rice protein. In vitro Simulated Gastrointestinal Digestion and Thermal Processing Herbicide-resistant transgenic rice Bar68-1 rice protein, non-transgenic parental control D68 rice protein, negative control casein, positive control soybean trypsin inhibitor and bovine serum albumin were purified by SDS-PAGE coagulation Gels electrophoresis to observe their degradation at each time point. The results showed that in the digestive stability test, the transgenic rice protein and the non-transgenic rice protein digested in 2 ~ 30 min, they can be digested in the simulated gastric and intestinal fluid; thermal stability test, transgenic rice protein and non Like the genetically modified rice protein, it still has good thermal stability after being heated for a certain time (15, 30, 60, 120 min) at a certain temperature (60, 75, 90 and 100 ℃). The results showed that the transgenic herbicide-resistant rice Bar68-1 had good thermal stability, digestible in simulated gastric juice and simulated intestinal fluid, and did not have allergen anti-digestion characteristics.