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目的：研究ｄＢｃＡＭＰ诱导分化成熟后的星状胶质细胞内脂多糖诱导型一氧化氮合酶活力的诱导表达情况．方法：采用荧光分光光度法和胍胺酸生成实验考察星状胶质细胞内一氧化氮合酶的活力，并通过免疫细胞化学和光学显微镜考察细胞的分化情况．结果：经ｄＢｃＡＭＰ诱导后，星状胶质细胞分化成星型细胞，ＧＦＡＰ表达增强．ｄＢｃＡＭＰ显著增强ＬＰＳ诱导产生的亚硝酸盐水平的提高；ｄＢｃＡＭＰ／ＬＰＳ共同诱导２４ｈ后，使ＮＯＳ活力比ＬＰＳ单独诱导提高４倍．ＬＮＡＭＥ，环己亚胺或放线菌素Ｄ都可分别抑制ｄＢｃＡＭＰ／ＬＰＳ的共同诱导作用．结论：ｄＢｃＡＭＰ可以上调ＬＰＳ对星状胶质细胞内ｉＮＯＳ的诱导作用．
OBJECTIVE: To study the induction of lipopolysaccharide-induced nitric oxide synthase (NOS) activity in astrocytes induced by dBcAMP-induced differentiation. Methods: The activity of nitric oxide synthase in astrocytes was investigated by fluorescence spectrophotometry and guanidine acid production assay. The differentiation of cells was examined by immunocytochemistry and light microscope. Results: After induced by dBcAMP, astrocytes differentiated into astrocytes and GFAP expression was enhanced. dBcAMP significantly increased the level of nitrite induced by LPS; when combined with dBcAMP / LPS, the activity of NOS was increased by 4 times than that induced by LPS alone. L NAME, cycloheximide or actinomycin D can inhibit the co-induced dBcAMP / LPS. Conclusion: dBcAMP can up-regulate the induction of iNOS in astrocytes by LPS.