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以糯玉米为原料,采用中性蛋白酶法预处理糯玉米制备淀粉,通过单因素实验,研究蛋白酶添加量、酶解时间、酶解温度、浸泡液pH、料液比对淀粉得率的影响,在单因素实验的基础上,采用5因素3水平的响应面法对反应条件进行优化,确定最佳工艺条件为：加酶用量5090.4U/g,酶解时间18.24h,酶解温度44.49℃,料液比1：4,pH7,在此条件下获得的淀粉得率为67.84%。“,”The waxy corn is used as material, the pretreating processes of waxy corn with the neutral protease method were researched. We studied the influence on yield of starch by the single factors： the amount of protease added, reaction time, reaction temperature, soaking solution pH, liquid material ratio. On the basis of single factor experiment, using five factors three levels of the response surface method to optimize reaction conditions, to determine the best technology conditions for： enzyme needed 5090.4 U/g; the ratio of gardenia 1：4; pH 7. Under these circumstances, the percentage of the starch extraction and residue protein is 67.84% and 2.14% respectively.