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目的探索雌激素受体α(ERα)阳性乳腺癌组织及乳腺纤维腺瘤组织中Mdm 2的表达,并探索MDM 2-si RNA对人乳腺癌MCF-7细胞增殖、克隆及凋亡的影响。方法 1回顾性收集笔者所在医院2012年6月至2015年10月期间的经病理组织学检查确诊的ERα阳性乳腺癌组织石蜡标本78例(乳腺癌组)及乳腺纤维腺瘤组织石蜡标本10例(乳腺纤维腺瘤组),采用免疫组化染色方法检测其Mdm 2的表达,并分析乳腺癌患者中Mdm 2表达与其临床病理特征的关系。2将MCF-7细胞分为MDM 2-si RNA组、阴性对照组及空白对照组,MDM 2-si RNA组和阴性对照组分别转染MDM 2-si RNA及无效干扰si RNA,空白对照组不加入任何试剂。检测3组细胞中Mdm 2的表达、细胞增殖率、克隆形成数量及凋亡率,并进行组间比较。结果 1乳腺纤维腺瘤组织中Mdm 2均呈阴性表达,表达阳性率为0(0/10);ERα阳性乳腺癌组织中Mdm 2阳性表达38例,阳性表达率为48.7%(38/78),高于乳腺纤维腺瘤组织(χ~2=12.357,P=0.000);ERα阳性乳腺癌组织中Mdm 2表达与患者的TNM分期和淋巴结转移数量均有关(P<0.050),TNM分期越晚、淋巴结转移数量越多,Mdm 2的表达阳性率越高。2MDM 2-siRNA组细胞的Mdm 2表达水平,转染后2、3及4 d时的细胞增殖率及细胞克隆形成数均低于空白对照组和阴性对照组(P<0.050),而细胞凋亡率高于空白对照组和阴性对照组(P<0.050),但空白对照组和阴性对照组转染后1、2、3及4 d时的细胞增殖率,Mdm 2表达水平,细胞克隆形成数及细胞凋亡率比较差异均无统计学意义(P>0.050)。结论ERα阳性乳腺癌患者中Mdm 2的表达情况是诊断乳腺癌的相对特异性标志物,靶向Mdm 2可能在ERα阳性乳腺癌患者的治疗中具有一定价值。
Objective To explore the expression of Mdm 2 in estrogen receptor α (ERα) positive breast cancer and breast fibroadenoma and explore the effect of MDM 2-si RNA on the proliferation, cloning and apoptosis of human breast cancer MCF-7 cells. Method 1 retrospectively collected from the hospital in our hospital from June 2012 to October 2015 period by histopathological examination of ERα positive breast cancer tissue samples of 78 cases (breast cancer group) and breast fibroadenoma tissue paraffin specimens in 10 cases (Breast fibroadenoma group). The expression of Mdm 2 was detected by immunohistochemical staining. The relationship between Mdm 2 expression and clinicopathological features was analyzed in breast cancer patients. The MCF-7 cells were divided into MDM 2-si RNA group, negative control group and blank control group, MDM 2-si RNA group and negative control group were transfected with MDM 2-si RNA and siRNA respectively, and blank control group Do not add any reagents. The expression of Mdm 2, cell proliferation rate, the number of clone formation and apoptosis rate were detected in 3 groups of cells, and compared among groups. Results Mdm 2 in breast fibroadenoma was negative and the positive rate was 0 (0/10). Mdm 2 positive expression in ERα positive breast cancer tissues was 38 (48.7%, 38/78) , Higher than that in breast fibroadenoma (χ ~ 2 = 12.357, P = 0.000). The expression of Mdm 2 in ERα positive breast cancer was correlated with TNM stage and lymph node metastasis (P <0.050) , The more lymph node metastasis, the higher the positive rate of Mdm 2 expression. Mdm 2 expression level in 2MDM 2-siRNA group was lower than that in blank control group and negative control group at 2, 3 and 4 days after transfection (P <0.050), while cell apoptosis The cell death rate was higher than that of blank control group and negative control group (P <0.050). However, the cell proliferation rate, Mdm 2 expression level and cell clone formation at 1, 2, 3 and 4 d after transfection in blank control group and negative control group There was no significant difference between the number of apoptotic cells and the apoptosis rate (P> 0.050). Conclusion The expression of Mdm 2 in ERα positive breast cancer patients is a specific marker for the diagnosis of breast cancer. The targeting of Mdm 2 may be of value in the treatment of patients with ERα positive breast cancer.