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目的研究肝癌基因治疗中的基因体内导人方法和途径。方法采用一种携带人白细胞介素2（IL－2）cDNA的质粒型EB病毒复制子表达载体，用Wistar大鼠建立点注射、门静脉注射和肝动脉注射加阻断法模型。每种模型分裸基因和脂质体-质粒注射两组，定期行免疫组化染色检测IL－2基因的表达。结果各组动物基因注射后3天即可见基因表达，3～7天时达到高峰，7天后开始下降。以裸基因肝动脉注射加栓塞表达效果最好。脂质体质粒注射不增加表达效率。结论基因直接导入结合手术和介入治疗方法可应用于肝癌的临床治疗。
Objective To study the methods and ways of gene transfer in hepatocellular carcinoma during gene therapy. Methods Plasmid EBV replicon expression vector carrying human interleukin 2 (IL-2) cDNA was used in this study. Point injection, portal vein injection and hepatic artery injection plus blockage model were established in Wistar rats. Each model was divided into two groups by naked gene and liposome-plasmid injection, and the expression of IL-2 gene was detected by immunohistochemistry on a regular basis. Results The gene expression was observed 3 days after gene injection in each group, peaked at 3-7 days, and then decreased at 7 days. To the bare gene hepatic artery embolization expression best. Liposomal plasmid injections did not increase the efficiency of expression. Conclusion Gene direct introduction combined with surgery and interventional therapy can be applied to the clinical treatment of liver cancer.