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①目的 探讨肢体缺血再灌注 (LIR)后内皮素 - 1 (ET - 1 )含量的变化及BQ - 1 2 3的干预作用。②方法 采用大鼠肢体缺血再灌注损伤模型 ,将Wistar大鼠随机分 8组 ,即 :对照组、单纯缺血组、再灌注 0 .5、2、4、6、1 0小时组与BQ - 1 2 3组 ,分别测定血浆LDH、CK活性、ET - 1水平及骨骼肌中MDA含量、ET - 1放免活性和组织湿 /干重比值。③结果 IR各组与对照组比较 ,血浆和骨骼肌的各项生化指标显著增高 (P <0 .0 1orP <0 .0 5) ;BQ - 1 2 3组与再灌注 4小时组比较 ,各项指标明显降低 (P <0 .0 1orP <0 .0 5)。④结论 ET - 1可能参与了肢体缺血再灌注损伤。BQ - 1 2 3可减轻肢体缺血再灌注损伤 ,对骨骼肌有保护作用。
Objective To investigate the changes of endothelin - 1 (ET - 1) content after limb ischemia - reperfusion (LIR) and the effect of BQ - 123 on it. Methods Wistar rats were randomly divided into 8 groups (control group, ischemia group, 0.5, 2, 4, 6 and 10 hours of reperfusion) and BQ - 1 2 3 group. The levels of plasma LDH and CK, the level of ET - 1, the content of MDA in skeletal muscle, the activity of ET - 1 and the ratio of wet to dry tissue were measured respectively. Results Compared with the control group, the biochemical indexes of plasma and skeletal muscle in IR group were significantly increased (P <0.01 or P <0.05), while those in BQ - 123 group and 4-hour reperfusion group Item index was significantly lower (P <0 1orP <0. 05). Conclusion ET - 1 may be involved in limb ischemia - reperfusion injury. BQ - 1 2 3 can reduce limb ischemia reperfusion injury and protect skeletal muscle.