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[Objective] To explore the application value of endophytic fungi isolated from Cephalotaxus oliveri Mast comprehensively.[Method] The fermentation products of strain gyzy-20 were extracted and analyzed by HPLC,and the producing process was optimized by orthogonal test.[Result] The metabolites of strain gyzy-20 contained homoharringtonine(HHT) whose content was affected by extraction duration largely.The optimized producing processes were as follows:carbon source of glucose,ultrasonic extraction duration of 5 min after fermenting for 10 d and extraction duration of 3 h after dichloromethane of twice the volumes was added in.The content of HHT reached as high as 0.26 μg/ml in fermentation broth under the optimum fermentation and extraction methods,which increased by more than 1.5 times.[Conclusion] The study can provide probability for producing homoharringtonine through microbial fermentation.
[Objective] To explore the application value of endophytic fungi isolated from Cephalotaxus oliveri Mast comprehensively. [Method] The fermentation products of strain gyzy-20 were extracted and analyzed by HPLC, and the producing process was optimized by orthogonal test. metabolites of strain gyzy-20 contained homoharringtonine (HHT) whose content was affected by extraction duration largely. The optimized producing processes were as follows: carbon source of glucose, ultrasonic extraction duration of 5 min after fermenting for 10 d and extraction duration of 3 h after dichloromethane of twice the volume was added in. The content of HHT reached as high as 0.26 μg / ml in fermentation broth under the optimum fermentation and extraction methods, which increased by more than 1.5 times. [Conclusion] The study can provide probability for producing homoharringtonine through microbial fermentation.