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【目的】明确2A型蛋白磷酸酶(PP2A)在玉米大斑病菌致病过程中的作用,为研发新型杀真菌制剂和探讨植物病害防治新策略奠定理论基础。【方法】利用不同浓度的PP2A特异性抑制剂——斑蝥素(cantharidin)处理玉米大斑病菌,研究在该抑制剂作用下玉米大斑病菌的菌落生长、分生孢子产量、孢子萌发、附着胞发育、黑色素合成及HT-毒素活性。【结果】随着斑蝥素浓度的增加,其对菌丝体生长的抑制作用也逐渐增强,当达到160μmol.L-1时,培养8 d的菌落平均直径仅为对照的41.7%;同时,处理组产孢量均高于对照组,160μmol.L-1斑蝥素处理组产孢量达到了对照组的15.5倍。分析斑蝥素对病菌分生孢子的萌发、附着胞形成及侵染的影响表明,上述3个阶段对斑蝥素的敏感性由强到弱依次为附着胞形成>分生孢子萌发>附着胞侵染;此外,对照组的胞内黑色素含量为0.13 g.L-1,处理组的胞内黑色素含量均高于对照组,且随着斑蝥素浓度的增加不断升高。【结论】PP2A特异性抑制剂斑蝥素对玉米大斑病菌菌落生长、附着胞发育具有抑制作用,而对分生孢子产生及胞内黑色素的合成具有一定的促进作用。
【Objective】 The purpose of this study was to clarify the role of type 2A protein phosphatase (PP2A) in pathogenicity of S. turcica and to lay a theoretical foundation for the development of new fungicide preparations and to explore new strategies for plant disease control. 【Method】 The S. turcica was treated with different concentration of PP2A inhibitor cantharidin to study the effects of the inhibitor on the growth, conidial production, spore germination, Development, melanin synthesis and HT-toxin activity. 【Result】 The results showed that with the increase of cantharidin concentration, the inhibitory effect on the growth of mycelia was gradually increased. When the concentration of cantharidin reached 160 μmol·L-1, the average diameter of colonies cultured for 8 days was only 41.7% of the control. At the same time, The amount of sporulation in the group was higher than that in the control group. The amount of sporulation in 160 μmol·L-1 cantharidin treatment group was 15.5 times that of the control group. The results showed that the sensitivity of cantharidin to conidia germination, appressorium formation and infection of pathogenic fungi was higher than that of cantharidin: attachment formation, conidiospore germination, ; In addition, the control group intracellular melanin content of 0.13 gL-1, intracellular melanin content were higher than the control group, and with the cantharidin concentration increased. 【Conclusion】 Cantharidin, a specific inhibitor of PP2A, has an inhibitory effect on the growth of colonization and attachment of S. turcica, but has some effects on the production of conidia and the synthesis of intracellular melanin.