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SOX家族与很多人类肿瘤的发生有关.对人类肺癌组织中的SOX4进行测序,发现该基因HMG-box区有突变发生并导致SOX4蛋白的107密码子发生突变.以三级结构已知的SOX2为模板,采用同源建模方法对突变和正常SOX4蛋白FMG-box进行建模.结果显示突变和正常SOX4蛋白的三级结构很相似,且突变导致107密码子位置的侧链结构不稳定.提示该位置的不稳定可能会影响突变SOX4蛋白侧链的弹性,并有可能影响相应蛋白的功能.表面静电分析显示SOX4蛋白C端有一个可能与其它小分子或蛋白质的相互作用位点的N/C腔.这些结果显示SOX4蛋白的上述空间结构可能与其活性与功能的调控有关,而SOX4突变可能与肺癌的发生和转移有关.
The SOX family is involved in the pathogenesis of many human cancers.Sequencing SOX4 in human lung cancer tissue revealed that there was a mutation in the HMG-box region of the gene and resulted in the mutation of 107 codons of SOX4 protein. Template was used to model the mutated and normal SOX4 protein FMG-box.The results showed that the tertiary structure of the mutant and normal SOX4 protein were similar and the mutation led to the instability of the side chain structure at 107 codon position. The instability at this site may affect the flexibility of the mutant SOX4 protein side chain and possibly affect the function of the corresponding protein.Analysis of surface electrostatic analysis shows that the C-terminal of SOX4 protein has a N / C cavity.These results suggest that the above spatial structure of SOX4 protein may be related to the regulation of its activity and function, and SOX4 mutation may be related to the occurrence and metastasis of lung cancer.