论文部分内容阅读
目的:观察细胞因子IL-12对Th17细胞分化的影响。方法:小鼠脾淋巴细胞经抗CD3单克隆抗体(mAb)和不同浓度的重组小鼠IL-12刺激,3d后使用ELISA方法观察培养物上清液中IL-17的产生情况。并使用细胞内细胞因子染色的方法,通过流式细胞术观察CD3mAb和重组小鼠IL-12刺激对Th1和Th17细胞分化的影响。结果:Th17细胞不分泌IFN-γ、IL-5、IL-10等细胞因子,不表达Foxp3,是一个独立的细胞亚群。不同浓度的重组小鼠IL-12可以诱导抗CD3mAb的T细胞分泌IFN-γ,并向Th1细胞方向分化。同时,IL-12可以抑制活化的T细胞分泌IL-17,抑制T细胞向Th17细胞分化。结论:IL-12可以抑制Th17细胞的分化。
Objective: To observe the effect of cytokine IL-12 on Th17 cell differentiation. Methods: The mouse splenic lymphocytes were stimulated with anti-CD3 monoclonal antibody (mAb) and different concentrations of recombinant mouse IL-12. The production of IL-17 in culture supernatants was observed by ELISA after 3 days. The effects of CD3 mAb and recombinant mouse IL-12 stimulation on the differentiation of Th1 and Th17 cells were observed by flow cytometry using intracellular cytokine staining. Results: Th17 cells did not secrete IFN-γ, IL-5, IL-10 and other cytokines, but did not express Foxp3, which was an independent cell subpopulation. Different concentrations of recombinant murine IL-12 induced the secretion of IFN-γ by anti-CD3 mAb T cells and differentiated into Th1 cells. At the same time, IL-12 can inhibit the secretion of IL-17 from activated T cells and inhibit the differentiation of T cells into Th17 cells. Conclusion: IL-12 can inhibit the differentiation of Th17 cells.