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过去一直用血糖浓度、尿糖量作为糖尿病控制指标,但这并不够。目前,掌握慢性糖尿病状态的指标HbA_1、HbA_(10)受到重视,临床上也受到了高度评价。HbA_1、HbA_(?):人的正常血红蛋白总量约90%为HbA(α_2β_2),约2%为HbA_2(α_2δ_2),约1%为HbF(α_2γ_2)。HbA 用色层法或电泳法,可将体内与糖非氧结合的HbA_1分离出来,进而再从HbA_1分离出HbA_1a_1、HbA_1a_2、HbA_1b、HbA_(?),除HbA_(?)以外,都和葡萄糖以外的糖相结合。HbA_1C 是HbA_1区带中含量最多的,约占60%。除HbA_(?)之外,在HbA(A_0A_2)中还有与葡萄糖相结合的部分,现在的HbA_(10)不过是与葡萄糖相结合的一部分。一般把稳定的HbA_(10)作为掌握慢性糖尿病状态和控制血糖的指标。测定方法:临床上多用微量柱层法或高速液相色谱法(HPLC)。柱层法简易、快速。此法只能测定HbA_1。但因受测定温度、缓冲液离子浓度、pH 等影响,故测定
In the past, blood glucose levels and urine sugar levels were used as indicators of diabetes control, but this is not enough. Currently, HbA_1 and HbA_ (10), which are indicators of chronic diabetic states, are valued and clinically highly valued. HbA_1, HbA_ (?): About 90% of human normal hemoglobin is HbA (α_2β_2), about 2% is HbA_2 (α_2δ_2) and about 1% is HbF (α_2γ_2). HbA can be separated from non-oxygenated HbA1 in vivo by chromatography or electrophoresis, and HbA_1a_1, HbA_1a_2, HbA_1b and HbA_ (?) Can be separated from HbA_1, HbA_ (?) And HbA_ Of sugar. HbA_1C is the most abundant in the HbA_1 zone, accounting for about 60%. In addition to HbA_ (?), HbA (A_0A_2) also has a portion that binds to glucose, and now HbA_ (10) is only part of the combination with glucose. Generally stable HbA_ (10) as an indicator of chronic diabetic status and control of blood glucose. Determination: Clinical use of micro-column or high-speed liquid chromatography (HPLC). Column method is simple and fast. This method can only determine HbA_1. However, due to the determination of temperature, buffer ion concentration, pH, etc., so the determination