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OsDREB1A是水稻冷信号传导途径中重要的转录因子.本研究将水稻日本晴OsDREB1A基因通过双酶切方法连接至MSC-TOPOⅡ载体,并重组构建到表达载体PGEX-4T-1,获得融合蛋白表达质粒PGEX-4T-1-OsDREB1A,转化表达菌E.coli BL21 (DE3) Condon Plus,诱导表达后通过亲和层析柱Glutathioneagarose 4B纯化获得了分子量大小正确的OsDREB1A-GST融合蛋白,EMSA检测发现低温18℃诱导纯化的融合蛋白可以在体外成功结合CRT/DRE顺式元件.为进一步研究OsDREB1A在冷信号传导中的生物学功能奠定了基础.“,”OsDREB1A is a key transcription factor in rice cold signal transduction pathway.Here we cloned the OsDREB1A gene into MSC-TOPO-Ⅱ vector by double enzyme digestion,then reconstructed it to prokaryotic expression vector PGEX-4T-1 by LR reaction,the recombinant vector pGEX4T-1-OsDREB1A-GST was transformed into E.coli.BL21 (DE3) Condon Plus.Recombinant protein OsDREB1A-GST was purified by glutathione agarose 4B after induce.The EMSA assay showed the OsDREB1A-GST protein induced under 18℃binding directly to CRT/DRE cis-element in OsPLDα1 promoter in vitro.These results provide foundations for the biological function study of OsDREB1A in cold signal transduction.