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用ＬＳＡＢ法对１２例肝细胞癌病人手术切除标本进行转化生长因子－β１（ＴＧＦ－β１）免疫染色，经计算机图象分析系统分析，发现肝癌组织的ＴＧＦ－β１表达明显低于癌旁肝组织，阳性染色面积（相对数），肝癌与癌旁肝组织分别为（９．９６±１０．７）％和（５６．６±２６．６２）％（Ｐ＜０．０１）。此外，外周血ＴＧＦ－β１水平测定（ＳＥＬＩＳＡ法）发现，健康志愿者组和肝硬化病人组外周血ＴＧＦ－β１水平明显高于肝癌病人（未治疗组），３组分别为（１８２．９２±９０．４５）μｇ／Ｌ，（１１９．５３±８７．７３）μｇ／Ｌ和（６２．３７±５１．５２）μｇ／Ｌ（Ｐ＜０．０１和Ｐ＜０．０５）；本研究提示肝癌细胞ＴＧＦ－β１表达降低可能与其细胞的转化和生长失控有关，也是其生物学特征之一。
The LSAB method was used to perform immunostaining for transforming growth factor-β1 (TGF-β1) in 12 surgically resected specimens of hepatocellular carcinoma. The expression of TGF-β1 in hepatocellular carcinoma was significantly lower than that in para-cancer liver tissue after analysis by computer image analysis system. The positive staining area (relative number), liver cancer and adjacent liver tissues were (9.96±10.7)% and (56.6±26.62)%, respectively (P<0.01). In addition, peripheral blood TGF-β1 levels (SELISA) found that the levels of TGF-β1 in peripheral blood of healthy volunteers and patients with liver cirrhosis were significantly higher than those of patients with liver cancer (untreated group). The three groups were (182.92±). 90.45) μg/L, (119.53±87.73) μg/L and (62.37±51.52) μg/L (P<0.01 and P<0.05); The decreased expression of TGF-β1 in hepatocellular carcinoma cells may be related to the loss of cell growth and control, and it is also one of its biological characteristics.