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采用阻抗谱分析法对体外培养人眼小梁网细胞进行实时在线检测.人眼眼压过高将导致青光眼病变,而小梁网对于眼压高低至关重要.制备了叉指式阵列微电极作为阻抗传感器敏感单元.提出了分析小梁网细胞生物行为特征的等效电路模型,通过仿真分析表明该电路模型可在不同频段区分人眼小梁网细胞外基质和细胞繁殖率等阻抗参数值.采用叉指式阻抗谱芯片对经地塞米松药物处理的小梁网细胞进行了实时在线监测,结果表明40 kHz时高浓度地塞米松对小梁网细胞增殖具有较大的抑制作用,该结论与AlamarBlue试剂法得到的结论一致.阻抗测试结果为糖皮质激素诱导的青光眼提供了一种合理的解释,认为该病变与正常细胞增殖率异常有关.
Real-time on-line detection of human trabecular meshwork cells by impedance spectroscopy was performed in real time.However, the high intraocular pressure of human eye led to the glaucoma lesion, and trabecular meshwork was very important for the intraocular pressure.An interdigital array microelectrode As the sensitive unit of impedance sensor.An equivalent circuit model for tracing the biological behavior of trabecular meshwork cells is proposed.The simulation results show that the circuit model can distinguish the impedance parameters of human trabecular meshwork extracellular matrix and cell propagation rate in different frequency bands The real-time on-line monitoring of trabecular meshwork cells treated with dexamethasone by using interdigitated impedance spectroscopy showed that dexamethasone at 40 kHz had a greater inhibitory effect on trabecular meshwork cell proliferation, The conclusion is consistent with the conclusion of the AlamarBlue reagent method that the impedance test provides a reasonable explanation for glucocorticoid-induced glaucoma, which is believed to be associated with abnormal proliferation of normal cells.