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Aim:To investigate the effects of panax notoginosides (PNS) on the proliferationof human hematopoietic stem/progenitor cells,and to explore the signaling path-way of the nuclear transcription factor of the glucocorticoid receptor (GR-NTF)initiated by PNS related with the proliferation.Methods:The human CD34~+ cellsand bone marrow nuclear cells were exposed to PNS at a concentration of 0,10,25,50,and 100 mg/L,respectively,in semi-solid culture system to observe colonyforming unite of all lineages,granulocyte,erythrocyte,and megakaryocyte (CFU-GEMM,CFU-GM,CFU-E,and CFU-MK).Three lineages of human hematopoieticcell lines,including granulocytic HL-60,erythrocytic K562,megakaryocytic CHRF-288,and Meg-01 cells were incubated with PNS at 20 mg/L for 14 d.Meanwhile,dexamethasone (Dex) was used as a positive control.The nuclear protein of thecells was analyzed by Western blotting with monoclonal antibodies against theamino or carboxyl terminus of GR-NTF.Electrophoretic mobility shift assay per-formed by using the ~(32)p-radiolabeled GR-NTF consensus oligonucleotide.Results:PNS promoted the proliferation of CD34~+ cells and significantly raised the colonynumbers of CFU-GEMM by 34.7%±16.0% over the non-PNS control (P<0.01).PNS also enhanced the proliferation of CFU-GM,CFU-E,and CFU-MK by 39.3%±5.7%,33.3%±7.3%,and 26.2%±3.2%,respectively.GR-NTF protein levels ofeither the amino or carboxyl terminus in K562,CHRF-288,and Meg-01 treated byPNS increased by 2.4-2.8 fold and 1.3-3.9 fold over the untreated cells.GR-NTFbinding activity,initiated by either PNS or Dex,was apparently elevated to formthe complex of GR-NTF with DNA as higher density bands in K562 and CHRF-288cells,and some activity appeared as a band in HL-60 cells induced by PNS.Conclusion:PNS displayed the action of hematopoietic growth factor-like or syn-ergistic efficacy to promote proliferation of human progenitor cells,may play arole in the upregulation of gene expression related to proliferation of hematopoi-etic cells through increasing the GR-NTF synthesis and its DNA binding activity.
Aim: To investigate the effects of panax notoginosides (PNS) on the proliferation of human hematopoietic stem/progenitor cells, and to explore the signaling path-way of the nuclear transcription factor of the glucocorticoid receptor (GR-NTF)initiated by PNS related with the proliferation.Methods:The human CD34~+ cellsand bone marrow nuclear cells were exposed to PNS at a concentration of 0,10,25,50,and 100 mg/L,respectively,in semi-solid culture system to observe colonyforming unite of all Lineages,granulocyte,erythrocyte,and megakaryocyte (CFU-GEMM,CFU-GM,CFU-E,and CFU-MK).Three lineages of human hematopoietic cell lines,including granulocytic HL-60,erythrocytic K562,megakaryocytic CHRF-288,and Meg -01 cells were incubated with PNS at 20 mg/L for 14 d.Meanwhile, dexamethasone (Dex) was used as a positive control. The nuclear protein of the cells was analyzed by Western blotting with monoclonal antibodies against the amino or carboxyl terminus of GR- NTF.Electrophoretic mobility shift assay per- Formed by using the ~(32)p-radiolabeled GR-NTF consensus oligonucleotide. Results: PNS promoted the proliferation of CD34~+ cells and significantly raised the colonynumbers of CFU-GEMM by 34.7%±16.0% over the non-PNS control ( P<0.01). PNS also enhanced the proliferation of CFU-GM, CFU-E, and CFU-MK by 39.3%±5.7%, 33.3%±7.3%, and 26.2%±3.2%,respectively.GR-NTF protein levels Ofeither the amino or carboxyl terminus in K562,CHRF-288,and Meg-01 treated byPNS increased by 2.4-2.8 fold and 1.3-3.9 fold over the untreated cells.GR-NTFbinding activity,initiated by either PNS or Dex,was apparently elevated To formthe complex of GR-NTF with DNA as higher density bands in K562 and CHRF-288cells, and some activity led as a band in HL-60 cells induced by PNS.Conclusion:PNS displayed the action of hematopoietic growth factor-like or syn -ergistic efficacy to promote proliferation of human progenitor cells,may play arole in the upregulation of gene expression related to proliferation of hematopoi-eti c cElls through increasing the GR-NTF synthesis and its DNA binding activity.