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目的 :研究热休克预处理对脑缺血再灌后热休克蛋白 70 (HSP70 )的表达影响及对缺血神经细胞损伤的保护作用。方法 :采用沙鼠短暂前脑缺血再灌损伤模型 ,光镜观察缺血再灌后神经细胞损伤情况 ,免疫组化方法检测脑缺血后不同时期额叶HSP70蛋白表达。结果 :沙鼠脑缺血后HSP70蛋白仅在再灌后 1d有少量表达 (P <0 .0 1) ;缺血神经细胞在再灌后第 7d大多出现损伤改变 ;热休克预处理能增加沙鼠脑缺血再灌后各期HSP70蛋白表达 (P <0 .0 1) ,明显减轻缺血神经细胞损伤 (P <0 .0 1)。结论 :热休克预处理能通过增加HSP70蛋白表达 ,减少缺血神经细胞损伤。
AIM: To investigate the effect of heat shock preconditioning on the expression of heat shock protein 70 (HSP70) and its protective effect on ischemic neuronal injury after cerebral ischemia-reperfusion. Methods: The model of gerbil short-term forebrain ischemia-reperfusion injury was used to observe the damage of neurons after ischemia-reperfusion. The expression of HSP70 in frontal lobes was detected by immunohistochemistry. Results: After gerbil ischemia, HSP70 protein was only slightly expressed on the 1st day after reperfusion (P <0.01). Most of the ischemic neuronal cells were damaged at 7th day after reperfusion. Heat shock pretreatment could increase the concentration of sand HSP70 protein expression in each stage of cerebral ischemia-reperfusion (P <0.01) significantly reduced the injury of ischemic neurons (P <0.01). Conclusion: Heat shock preconditioning can reduce the damage of ischemic neuronal cells by increasing HSP70 protein expression.