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目的 :了解精氨酸对肠粘膜屏障功能的影响。方法 :雄性 SD大鼠 30只 ,随机分为三组。精氨酸组予精氨酸0 .2 5 g腹腔注射 ,假手术组和肠梗阻组予生理盐水作对照 ,1次 / d,共 5 d。再手术将肠梗阻组、精氨酸组大鼠结扎回肠 ,造成单纯性机械性肠梗阻模型 ,假手术组仅作剖腹探查。术后 2 4h处死大鼠采集标本。结果 :1细菌移位率和移位数水平肠梗阻组和假手术组相比明显升高 ,精氨酸组和肠梗阻组相比明显减少。 2血浆内毒素水平肠梗阻组明显高于假手术组 ,精氨酸组与肠梗阻组相比下降。 3病理结果显示假手术组肠粘膜基本正常 ;肠梗阻组粘膜上皮脱落 ,绒毛坏死 ,粘膜下水肿 ,炎症细胞浸润 ;精氨酸组肠粘膜结构明显改善。结论 :肠梗阻可造成肠粘膜屏障功能损害 ,精氨酸可改善肠粘膜屏障功能 ,减少细菌移位。
Objective: To understand the effect of arginine on intestinal mucosal barrier function. Methods: Thirty male SD rats were randomly divided into three groups. Arginine group 0.2g arginine intraperitoneal injection, sham operation group and intestinal obstruction group with saline as a control, 1 times / d, a total of 5 d. Reoperation of the intestinal obstruction group, arginine group rats ligation of the ileum, causing a simple model of mechanical intestinal obstruction, sham-operated group only for exploratory laparotomy. The rats were sacrificed 24 hours after the operation. Results: 1 Bacterial translocation rate and number of translocation in intestinal obstruction group were significantly higher than those in sham-operation group, and arginine group and intestinal obstruction group were significantly decreased. 2 levels of plasma endotoxin in intestinal obstruction group was significantly higher than the sham group, arginine group and intestinal obstruction group decreased. 3 pathological results showed that the intestinal mucosa of sham operation group was basically normal; mucosal epithelial detachment, villus necrosis, submucosal edema and inflammatory cell infiltration in intestinal obstruction group; intestinal mucosa structure of arginine group was significantly improved. CONCLUSIONS: Intestinal obstruction can cause impaired intestinal mucosal barrier function. Arginine can improve intestinal mucosal barrier function and reduce bacterial translocation.