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目的：研究凋亡相关蛋白Ｂｃｌ－２、Ｂａｘ、Ｆａｓ和Ｆａｓ－Ｌ在非霍奇金淋巴瘤（ＮＨＬ）中的表达及意义。方法：应用免疫组化Ｓ－Ｐ法对５４例ＮＨＬ进行检测。结果：（１）Ｂｃｌ－２、Ｂａｘ、Ｆａｓ和Ｆａｓ－Ｌ阳性率分别为５０％、８３．８％、８８．９％和８８．７％。（２）反应性增生淋巴滤泡与肿瘤性滤泡的Ｂｃｌ－２表达方式相反。（３）Ｂｃｌ－２在Ｂ细胞性ＮＨＬ中的表达高于Ｔ细胞性ＮＨＬ。（４）低度恶性Ｂ细胞性ＮＨＬ中Ｂｃｌ－２的表达高于中～高度恶性组，而Ｆａｓ－Ｌ的表达强度低于中～高度恶性组。结论：Ｂｃｌ－２的免疫组化检测是鉴别淋巴结反应性增生与滤泡性淋巴瘤较可靠的诊断方法。Ｂｃｌ－２在Ｂ细胞性ＮＨＬ发病机制中比在Ｔ细胞性ＮＨＬ中可能发挥更重要的作用。对Ｂｃｌ－２和Ｆａｓ－Ｌ的免疫组化检测可能对估计ＮＨＬ恶性程度及预后有一定价值。
Objective: To study the expression and significance of apoptosis-related proteins Bcl-2, Bax, Fas and Fas-L in non-Hodgkin’s lymphoma (NHL). Methods: 54 cases of NHL were detected by immunohistochemical S-P method. Results: (1) The positive rates of Bcl-2, Bax, Fas, and Fas-L were 50%, 83.8%, 88.9%, and 88.7%, respectively. (2) The Bcl-2 expression patterns of reactive proliferative lymphoid follicles and neoplastic follicles are opposite. (3) The expression of Bcl-2 in B-cell NHL was higher than that in T-cell NHL. (4) The expression of Bcl-2 in low-grade B-cell NHL was higher than that in the medium-high-grade group. The expression level of Fas-L was lower than that in the medium to high-grade group. Conclusion: Immunohistochemical detection of Bcl-2 is a reliable method for differential diagnosis of lymph node reactive hyperplasia and follicular lymphoma. Bcl-2 may play a more important role in the pathogenesis of B-cell NHL than in T-cell NHL. Immunohistochemical detection of Bcl-2 and Fas-L may have some value in estimating the malignancy and prognosis of NHL.